SRP3238
EGF from rat
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
Epidermal Growth Factor, URG, Urogastrone
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
Recommended Products
biological source
rat
recombinant
expressed in E. coli
Assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
≤0.1 ng/mL
mol wt
6.2 kDa
packaging
pkg of 100 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
rat ... EGF(25313)
General description
Epidermal growth factor (EGF) is a potent growth factor and a cytoprotective peptide. Recombinant rat EGF is a 6.2kDa globular protein containing 54 amino acid residues, including 3 intramolecular disulfide-bonds.
Application
Epidermal growth factor (EGF) is used in hepatocyte culture to determine the optimal condition for survival and function of rat hepatocytes.
Biochem/physiol Actions
Epidermal growth factor (EGF) stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, inflammation and is involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α (transforming growth factor-α) and VGF (vaccinia virus growth factor).
Sequence
MNSNTGCPPS YDGYCLNGGV CMYVESVDRY VCNCVIGYIG ERCQHRDLRW WKLR
Physical form
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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The role of epidermal growth factor in prevention of oxidative injury and apoptosis induced by intestinal ischemia/reperfusion in rats.
Arda-Pirinci P
Acta Histochemica, 116(1), 167-175 (2014)
The epidermal growth factor receptor family: biology driving targeted therapeutics.
Wieduwilt MJ and Moasser MM
Cellular and Molecular Life Sciences, 65(10), 1566-1584 (2008)
Sohini Sen et al.
International journal of biological macromolecules, 143, 1009-1032 (2019-10-28)
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Epidermal growth factor improves survival and prevents intestinal injury in a murine model of pseudomonas aeruginosa pneumonia.
Dominguez JA
Shock, 36(4), 381-389 (2011)
Florence E Perrin et al.
PloS one, 5(12), e15914-e15914 (2011-01-07)
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