SRE0037
β-Glucuronidase from abalone
aqueous solution, β-glucuronidase ≥100,000 units/mL
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About This Item
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
form
aqueous solution
Quality Level
β-glucuronidase activity
≥100,000 units/mL
secondary activity
≤8,000 units/mL sulfatase
storage temp.
2-8°C
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Application
Beta-glucuronidase from abalone has been used as a standard to evaluate its performance in benzodiazepine liquid chromatography-mass spectrometry (LC-MS/MS)confirmation assays.
Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine.
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine.
Biochem/physiol Actions
Beta-glucuronidase is anenzyme present in several animals, plants, and bacteria. It catalyzes thehydrolysis of Beta-glucuronide conjugates and plant glucuronides.Beta-glucuronidase is a member of the glycosyl hydrolases (GHs) family 2 and is ahomo-tetrameric glycoprotein. It facilitates the removal of glucuronic acidsfrom glucuronides and aids in the liver metabolism enzymes for the excretion ofendo- and xeno-biotics.
Unit Definition
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).
Physical form
Supplied as an aqueous solution containing 0.85% NaCl and 0.02% sodium azide
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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To prove the relationship between the fluctuation in serum beta-glucuronidase level and hepatotoxicity, an inhibitor of beta-glucuronidase from G. lucidum was isolated and its hepatoprotective activity was investigated. The ether fraction of G. lucidum, which had potent beta-glucuronidase-inhibitory activity, protected
Articles
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
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