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SAB4301526

Sigma-Aldrich

Anti-phospho-mTOR (pSer2481) antibody produced in rabbit

affinity isolated antibody

Synonym(s):

FRAP, FRAP1, FRAP2, RAFT1, RAPT1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

288 kDa

species reactivity

rat, human, mouse

concentration

1.0 mg/mL

technique(s)

western blot: 1:500-1:1000 (Cell Lysate)

isotype

IgG

accession no.

NP_004949.1 

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer2481)

Gene Information

human ... MTOR(2475)

General description

Mechanistic target of rapamycin kinase (MTOR) is a serine/threonine kinase. mTOR belongs to the family of phosphatidylinositol kinase-related kinase (PIKK). mTOR exists as two different complexes, mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). In human chromosome, the gene MTOR is localized on 1p36.22.

Specificity

The antibody detects endogenous level of mTOR only when phosphorylated at Serine 2481.

Immunogen

Peptide sequence around phosphorylation site of Serine 2481(I-H-S(p)-F-I) derived from Human mTOR.

Application

Anti-phospho-mTOR (pSer2481) antibody produced in rabbit has been used in western blot analysis.

Biochem/physiol Actions

Mechanistic target of rapamycin kinase (MTOR) recognizes various intracellular and extracellular signals and processes them to regulate cell growth, metabolism, proliferation and survival. mTOR is also involved in mitochondrial respiration by activating oxidative phosphorylation. Deregulation of mTOR phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) complex signalling pathway, leads to leukaemogenesis. Mutations leading to aberrant expression of mTOR causes cancer cachexia. mTOR plays a key role in synaptic plasticity and autophagy. Polymorphism of mTOR is associated with brain malformation and various neuropsychiatric disorders like brain ischemia, autism, epilepsy, schizophrenia etc. mTOR is a key regulator of anabolic and catabolic processes. mTOR is activated by insulin or insulin like growth factor 1 (IGF1) and regulate PI3K/Akt/mTOR/tuberous sclerosis (TSC)/the small GTPase (guanidine triphosphatase) Ras (rat sarcoma) homologue enriched in brain (RHEB) signalling pathway. mTOR is a key regulator of lipid biogenesis by influencing lipogenic genes like sterol regulatory element-binding protein 1 (SREBP1). Dysregulation of mTOR is associated with type 2 diabetes and obesity.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Comparative integromics on Angiopoietin family members
Katoh Y and Katoh M
International Journal of Molecular Sciences, 17(6), 1145-1149 (2006)
Role of mTOR in Glucose and Lipid Metabolism
Mao Z and Zhang W
International Journal of Molecular Sciences, 19(7), 2043-2043 (2018)
Biological Aspects of mTOR in Leukemia
Mirabilii S, et al.
International Journal of Molecular Sciences, 19(8), 2396-2396 (2018)
mTOR and Tumor Cachexia
Duval AP, et al.
International Journal of Molecular Sciences, 19(8), 2225-2225 (2018)
mTOR-related brain dysfunctions in neuropsychiatric disorders
Ryskalin L, et al.
International Journal of Molecular Sciences, 19(8), 2226-2226 (2018)

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