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N8016

2-Nitrophenyl β-D-glucopyranoside

≥99% (TLC), powder

Synonym(s):

2-Nitrophenyl-beta-D-glucopyranoside, o-NPG

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About This Item

Empirical Formula (Hill Notation):
C12H15NO8
CAS Number:
2816-24-2
Molecular Weight:
301.25
NACRES:
NA.28
PubChem Substance ID:
24897848
UNSPSC Code:
12352204
EC Number:
220-568-5
MDL number:
MFCD00065048
Beilstein/REAXYS Number:
92206
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Product Name

2-Nitrophenyl β-D-glucopyranoside, powder

assay

≥99% (TLC)

Quality Segment

form

powder

solubility

water: 50 mg/mL

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9-,10+,11-,12-/m1/s1

InChI key

KUWPCJHYPSUOFW-RMPHRYRLSA-N

Application

2-Nitrophenyl β-D-glucopyranoside has been used as a substrate for glucosidase in protein assay and in a colorimetric assay to determine protein stability.

Biochem/physiol Actions

2-Nitrophenyl β-D-glucopyranoside serves as an artificial substrate for glucosidase.

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This Item
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2-Nitrophenyl β-D-glucopyranoside powder

Sigma-Aldrich

N8016

2-Nitrophenyl β-D-glucopyranoside

4-Nitrophenyl β-D-glucopyranoside ≥98% (TLC)

Sigma-Aldrich

N7006

4-Nitrophenyl β-D-glucopyranoside

Phenyl β-D-glucopyranoside ≥95.0%

Sigma-Aldrich

292710

Phenyl β-D-glucopyranoside

5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside ≥97%

Sigma-Aldrich

B4527

5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside

description

powder

description

≥98% (TLC)

description

≥95.0%

description

≥97%

assay

≥99% (TLC)

assay

≥98% (TLC)

assay

≥95.0%

assay

≥97%

solubility

water: 50 mg/mL

solubility

water: 50 mg/mL, clear to very slightly hazy

solubility

-

solubility

DMF: 50 mg/mL, clear, colorless to faintly yellow

Quality Level

300

Quality Level

300

Quality Level

100

Quality Level

200

form

powder

form

powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

−20°C

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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Questions

  1. hello, is this also a chromogenic substrate?

    1 answer

    1. Yes, this compound is considered to be a chromogenic beta-glucosidase substrate. Application testing is not performed on this product. Please see the links below to review publications that may be helpful.

      Characterization of a heat resistant ß-glucosidase as a new reporter in cells and mice
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2909947/

      Ion implantation treatment of beads for covalent binding of molecules: Application to bioethanol production using thermophilic beta-glucosidase
      https://www.sciencedirect.com/science/article/pii/S0141022913002019?via%3Dihub

      Helpful?

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