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M5904

Sigma-Aldrich

Mineral oil

for molecular biology, BioReagent, light oil

Synonym(s):

light mineral oil

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About This Item

CAS Number:
8042-47-5
EC Number:
232-455-8
MDL number:
MFCD00131611
UNSPSC Code:
12181504
NACRES:
NA.52

grade

for molecular biology

product line

BioReagent

form

light oil

technique(s)

PCR: suitable

color

colorless

refractive index

n20/D 1.467 (lit.)

density

0.84 g/mL at 25 °C (lit.)

suitability

suitable for overlaying aqueous reactions and centrifuge gradients

foreign activity

DNase, RNase, Protease, none detected

storage temp.

room temp

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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General description

Mineral oils are usually seen as a mixture of liquid hydrocarbons. It is derived from crude oil by distillation and refining.

Application

Mineral oil has been used:
  • to suspend the gels for capillary contraction assay
  • in polymerase chain reaction (PCR) reactions mixture to form a vapor barrier in order to prevent the evaporation of the reaction at elevated temperatures
  • as a continuous phase to prevent droplet coalescence in emulsification system

Mineral oil is suitable for use as an overlay to control evaporation and cross contamination of samples in a variety of molecular biology applications.

Biochem/physiol Actions

Mineral oil delivers a better insulation strength, when compared to air. It enhances the elimination of heat from the windings, when used in a power transformer.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Paraffin wax as a vapor barrier for the PCR.
Sparkman DR
Genome Research, 2(2), 180-181 (1992)
S Nathakarnkitkool et al.
Electrophoresis, 13(1-2), 18-31 (1992-01-01)
Capillary electrophoretic separations of double-stranded DNA fragments in the size range of 20-2200 base pairs were achieved in less than 20 min with the use of a Tris-borate buffer containing hydroxyethylcellulose. Analyses were carried out in both uncoated and phenylmethyl-coated
Electrical distribution and installation
Plant Engineer's Reference Book (2002)
C D Liapis et al.
Anticancer research, 25(3c), 2449-2452 (2005-08-06)
Carotid body (CB) is a highly specialized paraganglion originating from the neural crest ectoderm. CB paraganglion can be caused either by a genetic predisposition (hereditary paraganglia) or by chronic hypoxic stimulation. Germline mutations in any of the following genes: SDHD
Construction of a full-length enriched and a 5'-end enriched cDNA library using the oligo-capping method.
Yutaka Suzuki et al.
Methods in molecular biology (Clifton, N.J.), 221, 73-91 (2003-04-22)
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