Unfortunately, a Krebs-Ringer Bicarbonate Buffer without glucose is not available.
K4002
Krebs-Ringer Bicarbonate Buffer
With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
Bicarbonate Buffer Solution, Krebs Buffer, Krebs-Ringer Solution
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About This Item
NACRES:
NA.75
UNSPSC Code:
12161702
Pricing and availability is not currently available.
Quality Segment
form
powder
technique(s)
cell culture | mammalian: suitable
pH
6.1-6.7
solubility
water: 9.5 g/L, clear
storage temp.
2-8°C
General description
Krebs-Ringer Bicarbonate Buffer acts as a salt solution and plays an essential role in tissue culture. A salt solution provides the cells with water and inorganic ions. It also helps in maintaining the osmotic balance and ideal pH in the medium. This bicarbonate buffered isotonic saline contains magnesium, potassium, sodium, and phosphate ions.
Application
Preparation Note
Formulated to contain 9.5 grams of powder per liter of medium.
Supplement with 1.26 g/L sodium bicarbonate.
1 of 1
This Item | |||
|---|---|---|---|
| description With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture | description With sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture | description capsule | description Modified, without calcium chloride, magnesium sulfate and sodium bicarbonate, powder, suitable for cell culture |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable |
| Quality Level 500 | Quality Level 400 | Quality Level 200 | Quality Level - |
| form powder | form liquid | form capsule | form powder |
| storage temp. 2-8°C | storage temp. room temp | storage temp. - | storage temp. 2-8°C |
| pH 6.1-6.7 | pH - | pH 9.3-9.9 | pH - |
| solubility water: 9.5 g/L, clear | solubility - | solubility water, high purity: 1 capsule/100 mL | solubility - |
Storage Class
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Do you have a glucose free product?
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Product K4002 - Krebs-Ringer Bicarbonate Buffer, is without calcium chloride and sodium bicarbonate. In the reconstitution steps, you advice to add 1.26g sodium bicarbonate. Should we also add some calcium chloride? in which amount?
1 answer-
Product K4002 - Krebs-Ringer Bicarbonate Buffer does not contain calcium. The addition of calcium is not required for osmolarity. If you need to have calcium, you can add calcium chloride between 1.25 to 2.5 mM. You may want to check the osmolarity and make sure it is not too high.Kreb Henseleit Buffer has 1.25 mM calcium chlorideKreb's Buffer has 2.5 mM calcium chlorideKreb' Ringer Superfusion solution contains 2.0 mM calcium chloride.Please see the attached document for different Krebs' buffer recipes.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the formula of Product K4002, Krebs-Ringer Bicarbonate Buffer (KRB)?
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The formulation for KRB can be found on the data sheet.
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How should Product K4002, Krebs-Ringer Bicarbonate Buffer, be dissolved?
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Product No. K4002 is listed in a 10X1L package size, meaning that each of the ten containers has enough powder to make 1 Liter of KRB buffer. It can be dissolved in 1 Liter of either water or medium.
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