D5941

Drabkin′s reagent

for the determination of hemoglobin

• Synonym(s): Hemoglobin Quantitation Reagent
• NACRES: NA.25
• UNSPSC Code: 12352402
• Form: liquid

Properties

• Quality Segment: 200
• form: liquid

Description

General description

Drabkin′s reagent is used in measuring haemoglobin from blood samples. It comprises potassium ferricyanide, potassium cyanide and potassium dihydrogen phosphate as components. Potassium ferricyanide oxidizes haemoglobin to methemoglobin and then to cyanmethemoglobin. The cyanmethemoglobin levels is estimated at 530nm. It is a reliable method and can measure all haemoglobin forms except sulphaemoglobin.

Application

Drabkin′s reagent has been used for the quantitative colorimetric determination of blood haemoglobin in cerebral tissue to assess cerebral haemorrhage, in red blood cells to detect hemolysis and in contralateral and ipsilateral brain tissue homogenate.

Preparation Note

Contains sodium bicarbonate, potassium ferricyanide, and potassium cyanide.

Safety Information

• pictograms: GHS06,GHS08,GHS09
• signalword: Danger
• hcodes: H301 + H311,H319,H332,H373,H410
• target_organs: Thyroid
• supp_hazards: EUH032
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
• pcodes: P260 - P273 - P280 - P301 + P310 + P330 - P302 + P352 + P312 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - STOT RE 2