One unit of DNA equals 50 µg. Molecular biology grade TE buffer is suitable for diluting DNA.
D4889
Deoxyribonucleic acid sodium salt from Escherichia coli strain B
Genomic, unsheared
Synonym(s):
DNA
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About This Item
CAS Number:
UNSPSC Code:
41106310
eCl@ss:
32160414
EC Number:
297-226-7
NACRES:
NA.52
MDL number:
Biological source:
Escherichia coli
Form:
lyophilized powder
Solubility:
water: ~1 g/L
Storage temp.:
2-8°C
Pricing and availability is not currently available.
biological source
Escherichia coli
Quality Segment
grade
Molecular Biology
form
lyophilized powder
mol wt
>45 kb
solubility
water: ~1 g/L
storage temp.
2-8°C
General description
Escherichia coli strain B is most widely studied DNA after k-12. The genomes of E coli strain B and k-12 show differences in the IS sequence elements. Strain B have B-islands and express O-specific side-chain LPS. The product is unsheared genomic DNA.
Application
The DNAs are particularly useful for genomic analysis, including PCR, library construction in bacteriophage λ, and in heterologous pre-hybridization and hybridization protocols.
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used for:
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used for:
Biochem/physiol Actions
50% GC; Tm = 90.5 °C
Preparation Note
Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of approx. 2 units per mL. Approx. 20 A260 units per mg DNA. % GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
Analysis Note
Analyzed by gel electrophoresis and visualization after ethidium bromide staining, with single predominant band of 45 kB or greater.
Other Notes
DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
One unit will yield an A260 of 1.0 in 1.0 ml of 1 mM Tris-HCl, pH 7.5, with 1 mM NaCl and 1 mM EDTA (1 cm light path).
1 of 1
This Item | |||
|---|---|---|---|
| biological source Escherichia coli | biological source - | biological source - | biological source - |
| grade Molecular Biology | grade - | grade Molecular Biology | grade Molecular Biology |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form fibers | form liquid | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| solubility water: ~1 g/L | solubility water: 0.98-1.02 mg/mL, faintly hazy to hazy, colorless to light yellow | solubility - | solubility - |
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D4889 Sigma-Aldrich Deoxyribonucleic acid sodium salt from Escherichia coli strain B I have 1 Unit of this DNA. Just want to make sure if it =50ug. Also, Can i use TE buffer to dilute the DNA? thank you for your help.
1 answer-
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what is the absolute amount of DNA in this product is it 50µg?
1 answer-
This product is supplied in a unit size. 1 unit of any DNA = 50 µg.
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