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D4309

Sigma-Aldrich

REDTaq® DNA Polymerase

Taq for routine PCR with inert dye, 10X buffer included

Synonym(s):

DNA polymerase with loading dye, Taq DNA polymerase

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About This Item

MDL number:
MFCD01635818
UNSPSC Code:
12352204
NACRES:
NA.55

Quality Level

200

form

liquid

usage

sufficient for 1000 reactions
 sufficient for 250 reactions
 sufficient for 50 reactions

feature

dNTPs included: no
hotstart: no

concentration

1 unit/μL

technique(s)

PCR: suitable

color

red

input

purified DNA

suitability

suitable for PCR

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

General description

REDTaq® DNA Polymerase is a unique blend of Sigma′s quality Taq DNA Polymerase combined with an inert red dye. This dye enables quick visual confirmation of enzyme addition and reaction mixing. An aliquot of the samples (5-10 μl) can then be loaded directly onto an agarose gel for electrophoresis following PCR. The red dye migrates slightly faster than bromophenol blue at about the same rate as a 125 base pair fragment in a 1% agarose gel. Since no additional loading buffers are added to the reaction following PCR, reamplification is possible.
The red dye has no effect on automated or manual sequencing, restriction digestions or other downstream applications. However, if removing the dye is desired, this can easily be accomplished using any standard purification method.

Application

For routine PCR amplifications REDTaq® DNA Polymerase has been used in polymerase chain reaction (PCR) and reverse transcription-polymerase chain reaction (RT-PCR) analysis.

Features and Benefits

  • Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications.
  • Visual confirmation that not only has the enzyme been added, but that proper mixing has occurred.
  • No additional loading dyes are necessary. An aliquot can be taken directly from the reaction and loaded onto an agarose gel for electrophoresis.

Packaging

Provided with 10X reaction buffer containing MgCl2

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mutation or polymorphism of the CD20 gene is not associated with the response to R-CHOP in diffuse large B cell lymphoma patients
Sar A, et al.
Leukemia Research, 33(6), 792-797 (2009)
Mapping networks of protein-mediated physical interactions between chromitin elements.
Tiwari, V.K., and Baylin, S.B.
Current Protocols in Molecular Biology, 21-21 (2010)
Proteomics-based approach to elucidate the mechanism of antitumor effect of curcumin in cervical cancer
Madden K, et al.
Prostaglandins, Leukotrienes, and Essential Fatty Acids, 80(1), 9-18 (2009)
Innis, M.A., and Gelfand, D.H.
PCR Protocols: A Guide to Methods and Applications, 3-3 (1990)
Ovidiu Paun et al.
Methods in molecular biology (Clifton, N.J.), 862, 75-87 (2012-03-16)
Amplified fragment length polymorphism (AFLP) is a PCR-based technique that uses selective amplification of a subset of digested DNA fragments to generate and compare unique fingerprints for genomes of interest. The power of this method relies mainly in that it
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Introduction & Historical Timelines

Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

Introduction & Historical Timelines

Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

Introduction & Historical Timelines

Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

Protocols

Applications with REDTaq™

Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.

Standard PCR Protocol

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

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