D2650

Dimethyl sulfoxide

Hybri-Max^™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

• Synonym(s): DMSO
• Linear Formula: (CH₃)₂SO
• CAS Number: 67-68-5
• Molecular Weight: 78.13
• UNSPSC Code: 12191502
• NACRES: NA.75
• PubChem Substance ID: 24893703
• EC Number: 200-664-3
• Beilstein/REAXYS Number: 506008
• MDL number: MFCD00002089
• Assay: ≥99.7%
• Grade: Hybri-Max^™
• Technique(s): cell culture | hybridoma: suitable, cryopreservation: suitable
• Bp: 189 °C (lit.)
• Vapor pressure: 0.42 mmHg ( 20 °C)

Properties

• grade: Hybri-Max^™
• Quality Segment: 400
• vapor density: 2.7 (vs air)
• vapor pressure: 0.42 mmHg ( 20 °C)
• sterility: sterile-filtered
• product line: BioReagent
• assay: ≥99.7%
• form: liquid
• autoignition temp.: 573 °F
• feature: subscription eligible
• expl. lim.: 42 %, 63 °F
• technique(s): cell culture | hybridoma: suitable, cryopreservation: suitable
• impurities: endotoxin, tested, ≤0.1% water; water
• refractive index: n20/D 1.479 (lit.)
• bp: 189 °C (lit.)
• mp: 16-19 °C (lit.)
• solubility: H₂O: miscible (completely)
• density: 1.10 g/mL (lit.)
• SMILES string: CS(C)=O
• InChI: 1S/C2H6OS/c1-4(2)3/h1-2H3
• InChI key: IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Description

Application

DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines; embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS).

Packaging

This product features a liner in the cap designed to prevent leakage. In rare cases, the liner may remain on the neck of the bottle after cap removal. This does not affect the product′s integrity. If this occurs, simply reattach the liner to the cap.

Glass ampoule packaging is now available under a new product number, D2653

100 mL in amber bottle

Other Notes

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This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is sterile filtered (0.2 micron) and tested for endotoxin levels.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: 188.6 °F - closed cup
• flash_point_c: 87 °C - closed cup
• ppe: Eyeshields, Gloves, type ABEK (EN14387) respirator filter