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Key Documents

Detroit 551

86082802, human skin (embryonic), Fibroblast

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About This Item

UNSPSC Code:
41106514

product name

Detroit 551, 86082802

biological source

human skin (embryonic)

growth mode

Adherent

karyotype

2n = 46

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human Caucasian embryo skin

Cell Line Description

Derived from a skin biopsy of a normal Caucasian female embryo. Cells may be considered normal and may serve as controls for study of cells having mutant chromosomes. Cells support growth of herpes viruses and SV40 virus can induce transformation.

Application

Chromosome and virus studies (Normal control): herpesviruses, SV40

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 12,13
D5S818: 12,13
D7S820: 10,12
THO1: 9,9.3
TPOX: 8
vWA: 18

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Finite lifespan; at most 25 subcultures from original tissue.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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