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IMR 32

86041809, human nerve, Fibroblast/neuroblast

Synonym(s):

IMR32 Cells, Institute for Medical Research-32 Cells

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About This Item

UNSPSC Code:
41106514

product name

IMR 32, 86041809

biological source

human nerve

growth mode

Adherent

karyotype

2n = 46

morphology

Fibroblast/neuroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

metastasis

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human Caucasian neuroblastoma

Cell Line Description

Established from an abdominal mass occurring in a 13 month old Caucasian male. The tumour was diagnosed as a neuroblastoma with two rare areas of orgonoid differentiation. The culture is a mixture of two morphologically distinct cell types, a small neuroblast-like cell and the other a large hyaline fibroblast. IMR 32 is partially resistant to polioviruses type 3 and highly resistant to ECHO-II. It is susceptible to Coxsackie B3.

Application

IMR 32 has been used to study the transmigration of cells through a human in vitro blood-cerebrospinal fluid barrier (BCSFB) model.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. The cells have a low plating efficiency and cell attachment can take up to 2-4 days after resuscitation. Cells easily detach. May n

Other Notes

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Certificates of Analysis (COA)

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