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HF19

85011418, human lung, Fibroblast-like

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About This Item

UNSPSC Code:
41106514

product name

HF19, 85011418

biological source

human lung

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human foetal lung fibroblast

Cell Line Description

HF19 has been established from a 14 week old female foetus. The cells are heterozygous for thymidine kinase and have been used for studies of radiation-induced DNA damage.

Application

DNA repair studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 9,13
D16S539: 13,14
D5S818: 11,12
D7S820: 11,12
THO1: 6,7
TPOX: 8,11
vWA: 14,18

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-4x10,000 cells /cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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