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C6854

Cathepsin L from human liver

≥0.5 units/mg protein, solution

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About This Item

CAS Number:
60616-82-2
UNSPSC Code:
12352200
NACRES:
NA.32
EC Number:
3.4.22.15 (BRENDA, IUBMB)
MDL number:
MFCD00163235
Specific activity:
≥0.5 units/mg protein
Biological source:
human liver
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biological source

human liver

Quality Segment

200

form

solution

specific activity

≥0.5 units/mg protein

technique(s)

activity assay: suitable

pH range

4.5—5.5

UniProt accession no.

P07711

application(s)

pharmaceutical

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CTSL1(1514)

General description

The cathepsin L (CTSL) gene is mapped to human chromosome 9q21.33. It encodes a lysosomal cysteine proteinase that belongs to the peptidase C1 family. The enzyme is a dimer of a heavy (175 amino acids) and a light chain (42 amino acids) linked by disulfide bonds.

Application

Cathepsin L (CTSL) from human liver has been used:
  • to digest mutant transforming growth factor β-induced (TGFBIp) and LC3 (autophagosomes marker) proteins in vitro
  • to digest the pH-dependent fibril of a pre-melanosomal protein (Pmel17) repeat domain (RPT) isoform and study the effect of pH on sRPT aggregation kinetics by tryptophan fluorescence
  • to analyze the inhibition of CTSL by small molecules or drugs in a cell-free system

Biochem/physiol Actions

Cathepsin L (CTSL) exhibits endopeptidase activity and breaks peptide bonds with aromatic residues. This enzyme is functional at pH 3.0–6.5 with thiol compounds. Elastin, collagen, and α-1 protease inhibitors act as substrates for cathepsin L (CTSL). CTSL cleaves several proteins including enzymes, receptors, and transcription factors. It is involved in antigenic peptide production and controls B-cells homeostasis. It is associated with myofibril necrosis and tumor progression. Increased expression of CTSL promotes metastasis and relates to poor prognosis in cancer patients. Increased expression of CTSL is observed in breast cancer. CTSL is implicated in glioblastoma multiforme (GBM), middle east respiratory syndrome (MERS), gingival overgrowth, and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It has a higher specific activity than cathepsin B and H in the degradation of a variety of physiological protein substrates.

Physical form

Solution in in 20 mM malonate, pH 5.5, 1 mM EDTA, and 400 mM NaCl.

Other Notes

One unit will hydrolyze 1.0 μmole of Z-Phe-Arg-AFC per minute at pH 5.5 at 25 °C.

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This Item
C8571C4428SRP0291
Cathepsin L from human liver ≥0.5 units/mg protein, solution

Sigma-Aldrich

C6854

Cathepsin L from human liver

Cathepsin B from human liver buffered aqueous solution, ≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.)

Sigma-Aldrich

C8571

Cathepsin B from human liver

Cathepsin G from human leukocytes lyophilized powder, ≥60 units/mg protein (Bradford)

Sigma-Aldrich

C4428

Cathepsin G from human leukocytes

Cathepsin L Active human recombinant, expressed in FreeStyle™ 293-F cells, ≥90% (SDS-PAGE)

Sigma-Aldrich

SRP0291

Cathepsin L Active human

technique(s)

activity assay: suitable

technique(s)

-

technique(s)

-

technique(s)

inhibition assay: suitable

biological source

human liver

biological source

human liver

biological source

human leucocytes

biological source

human

Gene Information

human ... CTSL1(1514)

Gene Information

human ... CTSB(1508)

Gene Information

human ... CTSG(1511)

Gene Information

human ... CTSL(1514)

specific activity

≥0.5 units/mg protein

specific activity

≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.)

specific activity

≥60 units/mg protein (Bradford)

specific activity

≥3900 pmol/min-μg

form

solution

form

buffered aqueous solution

form

lyophilized powder

form

aqueous solution

application(s)

pharmaceutical

application(s)

-

application(s)

-

application(s)

life science and biopharma

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

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Questions

  1. Hello, What is the protocol to bring this up in solution to have a concentration of 0.5 units/µL? Does units mean µg where the amount needed for performing digestions? Is there a protocol associated with this?

    1 answer

    1. This product is supplied as a solution. The minimum activity for each lot will be ≥0.5 units/mg protein. The specific activity of each lot is reported on the Certificate of Analysis. See the link below to review a sample Certificate:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/C6854-25UGSLCR2105.pdf

      The product is supplied in 20 mM malonate buffer, pH 5.5, with 1 mM EDTA and 400 mM NaCl. One unit is defined as the amount of Cathepsin L that hydrolyzes 1 uM Z-PHE-Arg-AFC per minute at 22 - 25 °C, in 400 mM Sodium Acetate, pH 5.5, containing 4 mM EDTA and 8 mM DTT.

      Please access the product datasheet at the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/130/380/c6854dat.pdf

      Helpful?

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