C-14036

Human Cardiac Fibroblasts (HCF)

1 million cells per pellet

• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human heart (ventricles)
• Morphology: (fibroblast)
• Relevant disease(s): cardiovascular diseases

Properties

• biological source: human heart (ventricles)
• packaging: pkg of 1,000,000 cells
• morphology: (fibroblast)
• application(s): cell analysis (DNA, RNA, and protein)
• relevant disease(s): cardiovascular diseases
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Cells are dissolved in RNAlater for subsequent RNA, DNA, or protein analysis. Note that cell pellets cannot be revived.

Application

Primary Human Cardiac Fibroblasts (HCF) are isolated from the ventricles of the adult heart. They play a central role in the maintenance of the extracellular matrix in the normal heart and the synthesis of growth factors and cytokines. Under pathophysiological conditions, cardiac fibroblasts are involved in scar formation after myocardial infarction, cardiac fibrosis, and cardiac hypertrophy. HCF can be used to study such processes in vitro.

Biochem/physiol Actions

Heart

Physical form

Pellet is dissolved in RNAlater^® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.

Preparation Note

Click here for more information.

Analysis Note

Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.

Legal Information

RNAlater is a registered trademark of Thermo Fisher Scientific, Inc.

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable