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B9285
5-Bromo-2′-deoxyuridine
≥99%, synthetic (organic), powder
Synonym(s):
5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR
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About This Item
Empirical Formula (Hill Notation):
C9H11BrN2O5
CAS Number:
Molecular Weight:
307.10
NACRES:
NA.51
PubChem Substance ID:
UNSPSC Code:
41106305
EC Number:
200-415-9
MDL number:
Beilstein/REAXYS Number:
30395
Assay:
≥99%
Biological source:
synthetic (organic)
Form:
powder
Solubility:
NH4OH: 0.1 M at 20 °C, clear, colorless
Storage temp.:
−20°C
Pricing and availability is not currently available.
Product Name
5-Bromo-2′-deoxyuridine, BioUltra, ≥99%
biological source
synthetic (organic)
Quality Segment
product line
BioUltra
assay
≥99%
form
powder
impurities
≤0.005% Phosphorus (P), ≤0.1% Insoluble matter
ign. residue
≤0.1%
mp
191-194 °C (dec.) (lit.)
solubility
NH4OH: 0.1 M at 20 °C, clear, colorless
cation traces
Al: ≤0.0005%, Ca: ≤0.01%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.0005%, NH4+: ≤0.05%, Na: ≤0.05%, Pb: ≤0.001%, Zn: ≤0.0005%
storage temp.
−20°C
SMILES string
OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O
InChI
1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
InChI key
WOVKYSAHUYNSMH-RRKCRQDMSA-N
General description
5′-Bromo-2-deoxyuridine (BrdU) is a carcinogen that may be absorbed by skin or inhalation. BrdU solutions are sensitive to light.
Application
5-Bromo-2′-deoxyuridine has been used:
- to label proliferating cells in pregnant mice in cell proliferation assay
- to study the time course of cell proliferation at various times following ischemia, to confirm re-entry of MKI67+ cells in cell cycle to germ cells
- in 5′-Bromo-2′Deoxyuridine (BrdUrd) staining of fibroblasts
Biochem/physiol Actions
5-Bromo-2′-deoxyuridine (5-BrdU) is a thymidine analogue which is incorporated into DNA. 5-BrdU is routinely and extensively used to measure DNA synthesis and to label dividing cells. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation.
Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.
5′-Bromo-2-deoxyuridine (BrdU) staining is predominantly used to observe the multiplication of tumor cells and other tissues in vivo.
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This Item | |||
|---|---|---|---|
| biological source synthetic (organic) | biological source synthetic (organic) | biological source - | biological source synthetic (organic) |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| form powder | form powder | form solid | form powder |
| assay ≥99% | assay ≥99% (HPLC) | assay ≥98% (HPLC) | assay ≥90% |
| storage temp. −20°C | storage temp. −20°C | storage temp. 15-25°C | storage temp. −20°C |
| solubility NH4OH: 0.1 M at 20 °C, clear, colorless | solubility DMSO: 50 mg/mL, clear, colorless to very faintly yellow | solubility water: 10 mg/mL, 0.1 M NaOH: 50 mg/mL, DMSO: 50 mg/mL | solubility water: 50 mg/mL, clear, colorless to faintly yellow |
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signalword
Danger
hcodes
Hazard Classifications
Muta. 1B - Repr. 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 2
ppe
Eyeshields, Gloves, type N95 (US)
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What is the Department of Transportation shipping information for this product?
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What is the shelf life of Product No. B9285 (5-bromo-2´-deoxyuridine)?
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Sigma-Aldrich does not assign an expiration date to this product; it is guaranteed for one year from the date of shipment.
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Can Product B9285, 5-Bromo-2´-deoxyuridine, be injected into animals?
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Sigma has not injected animals with solutions of this compound, so we do not have a stepwise procedure for you. However, this application has been reported in the literature. Please see the cited references below, which you may find useful. Several references cite the use of Product No. B9285 in their protocols.1. Yin, X-X, et al., Expression of active caspase-3 in mitotic and postmitotic cells of the rat forebrain. J. Comp. Neurol., 433(1), 4-22 (2001). Injected into mice at 50 mg/kg.2. Dackor, R.T., et al., Hydrops Fetalis, Cardiovascular Defects, and Embryonic Lethality in Mice Lacking the Calcitonin Receptor-Like Receptor Gene. Mol. Cell. Biol., 26(7), 2511-2518 (2006). Injected into mice at 100 mg/kg.3. Farris, S.M., et al., Larval and Pupal Development of the Mushroom Bodies in the Honey Bee, Apis mellifera. J. Comp. Neurol., 414, 97-113 (1999). A 25 mg/mL solution was injected into bee larvae, prepupae, and pupae.4. Gaiano, N., et al., Radial Glial Identity Is Promoted by Notch1 Signaling in the Murine Forebrain. Neuron, 26(2), 395-404 (2000). A 20 mg/mL solution was injected into mice.
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In what solvents is Product B9285, 5-Bromo-2´-deoxyuridine, soluble?
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Solubility information for 5-bromo-2´-deoxyuridine can be found on the product information sheet (under Documents, above).
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For how long is Product B9285, 5-Bromo-2´-deoxyuridine, stable in solution?
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5-Bromo-2´-deoxyuridine is stable in solution for 6 months when stored -20°C.
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