B8041

Biglycan from bovine articular cartilage

essentially salt-free, lyophilized powder

• UNSPSC Code: 12352202
• NACRES: NA.75
• MDL number: MFCD00676525
• Form: essentially salt-free, lyophilized powder
• Biological source: bovine articular cartilage
• Mol wt: 200-350 kDa

Properties

• biological source: bovine articular cartilage
• Quality Segment: 200
• form: essentially salt-free, lyophilized powder
• mol wt: 200-350 kDa
• packaging: glass bottle of 0.5 mg
• technique(s): cell culture | mammalian: suitable
• solubility: water: soluble ≥2.00 mg/mL, clear, colorless
• UniProt accession no.: P21809
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: cow ... BGN(280733)

Description

General description

proteoglycan consisting of a 45 kDa core protein and two chrondroitin/dermatan sulfate glycosaminoglycan chains

Biochem/physiol Actions

Biglycan interacts with collagen type I and II, fibronectin and TGF-β. In a low ionic strength (less than 3 mM phosphate) buffer, Biglycan binds more strongly to collagen type I. At higher ionic strengths, Biglycan will instead increase the inhibition effect of TGF-β on osteoclast proliferation at a concentration of 4-20 mg/mL. It also functions to prevent the attachment of CHO cells to fibronectin, with a 50% inhibition at 17-21 mg/mL. As an extracellular component, Biglycan binds TGF-β 1, facilitates bone mineralization and supports osteoblast differentiation thru BMP-4 binding.

Preparation Note

This product is extracted from articular cartilage, chematographically purified, dialyzed against water, and 0.2 μm filtered prior to lyophilization. Once lyophilized, this powder is essentially salt-free. The product is soluble in water at 2 mg/mL.

Other Notes

Biglycan is a 200-350 kDa proteoglycan consisting of a 45 kDa core protein and two chondroitin/dermatan sulfate glycosaminoglycan chains.

Disclaimer

Store this product at -20°C. Stored as supplied, this powder shows little decomposition in 3 years when stored properly.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)