B7653
Monoclonal Anti-Biotin antibody produced in mouse
clone BN-34, ascites fluid
Synonym(s):
Monoclonal Anti-Biotin
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Recommended Products
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
BN-34, monoclonal
contains
15 mM sodium azide
technique(s)
capture ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: 1:4,000
isotype
IgG1
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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General description
Biotin is a water-soluble vitamin. It is also called as vitamin B8. Biotin is a bicyclic compound that has a uredo ring and a tetrahydrothiophene ring.
Monoclonal Anti-Biotin recognizes the free biotin molecule and biotin conjugated to various immunoglobulins in ELISA and immunohistochemical techniques. Specificity was verified by using biotinylated goat antibodies reactive against human and rabbit antigens coated on microwell plates.
The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immunohistochemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.
Immunogen
Biotin-KLH conjugate
Biotinylated keyhole limpet hemocyanin (KLH)
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-Biotin antibody has been used in proximity ligation assay (PLA), cell surface biotinylation assay and western blotting.
The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
Utilized in a variety of applications including blotting, immunocytochemistyr, insitu nucleic acid hybridization, ELISA, fluorescent activated cell-sorting (FACS) and electron microscopy.Titer is approximately 1:2000 by ELISA but should be optimized by the individual user for their application of choiceAn essential vitamin, biotin is required by living organisms and cells in culture. Avidin′s high affinity for biotin is often exploited in immuno-based assays. While standard assay methods using the avidin-biotin enzyme complex will suffice for most studies, there are occasions when enhanced sensitivity is needed to detect smaller amounts of antigen or localize low density antigens in histologic sections. Conventional immunoassay methods are improved by the use of Monoclonal Anti-Biotin which enhances the sensitivity of avidin-biotin immunoassays by bridging a second layer of avidin-biotin-enzyme complex. This antibody can be used in many other applications where biotin can be introduced as a target label.
Monoclonal Anti-Biotin antibody produced in mouse is suitable for ELISA at a working dilution of 1:4000 and for western blotting.
Monoclonal Anti-Biotin antibody produced in mouse is suitable for ELISA at a working dilution of 1:4000 and for western blotting.
Biochem/physiol Actions
Biotin may participate in the replication and transcription of DNA. It acts as a coenzyme in the fatty acid biosynthesis, glyconeogenesis, and catabolism of several branched-chain amino acids and odd-chain fatty acids.
Preparation Note
Developed in BALB/c mice immunized with biotinylated KLH. Mouse myeloma cells and splenocytes are fused together to form a BN-34 hybridoma.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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