Potassium buffer is not required. For assay purposes, this product is reconstituted in potassium phosphate buffer, pH 6.0, at 0.2 mg/ml, Solutions may also be prepared 40 mM Tris HCL, pH 7.5, containing 50 mM NaCl and 50% glycerol. This stock may be stored for extended periods at –20 °C. Other sources indicate that this enzyme may also be prepared in water containing 0.01% BSA and TBE buffer. These options have not been validated. Please see the link below to review a publication that may be of interest:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817930/
A6306
Agarase from Pseudomonas atlantica
lyophilized powder, ≥5,000 units/mg protein (Lowry)
Synonym(s):
β-Agarase, Agarose 4-glycanohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
42040102
NACRES:
NA.26
MDL number:
Specific activity:
≥5,000 units/mg protein (Lowry)
Biological source:
bacterial (Pseudomonas atlantica)
Pricing and availability is not currently available.
biological source
bacterial (Pseudomonas atlantica)
Quality Segment
form
lyophilized powder
specific activity
≥5,000 units/mg protein (Lowry)
storage temp.
2-8°C
Physical form
Product is formulated with phosphate buffered salts, bovine serum albumin and Agarase. Total protein content ranges between 10 – 30% w/w.
Other Notes
One unit will produce 1.0 μg of reducing sugar (measured as D-galactose) from agar per min at pH 6.0 at 40 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
1 of 1
This Item | |||
|---|---|---|---|
| biological source bacterial (Pseudomonas atlantica) | biological source - | biological source - | biological source - |
| specific activity ≥5,000 units/mg protein (Lowry) | specific activity - | specific activity - | specific activity - |
| form lyophilized powder | form buffered aqueous suspension | form solution | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Will this enzyme work in water or PBS, or must it be a potassium buffer (for which not further details are provided in the spec sheet)?
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