37294

Dihydrofolate Reductase from bovine liver

~8 U/mL, ammonium sulfate suspension, off-white

• Synonym(s): Tetrahydrofolate Dehydrogenase
• CAS Number: 9002-03-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 1.5.1.3 (BRENDA, IUBMB)
• MDL number: MFCD00130955
• Specific activity: ≥6 U/mg protein
• Biological source: bovine liver
• Concentration: ~8 U/mL

Properties

• biological source: bovine liver
• form: ammonium sulfate suspension
• specific activity: ≥6 U/mg protein
• concentration: ~8 U/mL
• color: off-white
• density: 1.2 g/mL at 20 °C
• storage temp.: 2-8°C

Description

Application

Dihydrofolate reductase (DHFR) is involved in the synthesis of purines, thymidine and glycine in folate metabolism. DHFR, from bovine liver, is used for methotrexate analysis . DHFR is useful to study various cancers such as sarcomas, leukemias and head and neck cancers .

Biochem/physiol Actions

Dihydrofolate reductase (DHFR) reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as an electron donor. The binding of antitumor agents, such as methotrexate, to DHFR prevents the formation of reduced folates, which are essential for DNA synthesis .

Physical form

Suspension in 3.6 M ammonium sulfate solution, pH 7.0

Other Notes

1 U corresponds to the amount of enzyme which converts 1 μmol 7,8-dihydrofolate and NADPH to 5,6,7,8-tetrahydrofolate and NADP per minute at pH 6.5 and 25 °C

In folate metabolism for the synthesis of purines, thymidine and glycine

Sales restrictions may apply

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves