D2935

Decarboxylase broth base, Moeller

NutriSelect^® Plus, powder, nonselective and differential for Enterobacter spp., pack of 500 g, suitable for microbiology

• Synonym(s): Moeller Decarboxylase Broth, Moeller Decarboxylase Broth Base
• NACRES: NA.85
• UNSPSC Code: 41171606
• Manufacturer/tradename: NutriSelect^® Plus
• Form: powder
• Suitability: nonselective and differential for Citrobacter spp., nonselective and differential for Enterobacter spp., nonselective and differential for Escherichia coli, nonselective and differential for Klebsiella spp., nonselective and differential for Proteus spp., nonselective and differential for Salmonella spp., nonselective and differential for Serratia spp., nonselective and differential for Shigella spp., nonselective and differential for coliforms, nonselective and differential for enterobacteriaceae
• Application(s): clinical testing; environmental; food and beverages, microbiology

Properties

• sterility: non-sterile
• Quality Segment: 200
• product line: BioChemika
• form: powder
• shelf life: limited shelf life, expiry date on the label
• packaging: pack of 500 g
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbe id | specific enzyme detection: suitable, microbiological culture: suitable
• color: greenish yellow
• final pH: 6.0±0.2 (25 °C)
• application(s): clinical testing; environmental; food and beverages, microbiology
• suitability: nonselective and differential for Citrobacter spp., nonselective and differential for Enterobacter spp., nonselective and differential for Escherichia coli, nonselective and differential for Klebsiella spp., nonselective and differential for Proteus spp., nonselective and differential for Salmonella spp., nonselective and differential for Serratia spp., nonselective and differential for Shigella spp., nonselective and differential for coliforms, nonselective and differential for enterobacteriaceae

Description

General description

Decarboxylase broth base, Moeller is a dehydrated culture medium designedfor the differentiation of Gram-negative enteric bacteria based on theirability to decarboxylate specific amino acids. The medium follows the Moellerformulation, which contains peptone, beef extract, glucose, pyridoxal (as acofactor), and bromocresol purple as a pH indicator, but no amino acids bydefault; appropriate L‑amino acids (L‑arginine, L‑lysine, or L‑ornithine) are added separately depending on the test required. Wheninoculated and overlaid with mineral oil to ensure anaerobic conditions,decarboxylase-positive organisms produce alkaline amines that raise the pH andshift the indicator from yellow (acidic) back to purple (alkaline), allowingdistinction between decarboxylase-positive and -negative strains.

Application

Decarboxylase broth base, Moeller is used primarily in clinical and industrial microbiology laboratories for biochemical identification of Enterobacteriaceae and other Gram‑negative rods through detection of lysine, ornithine, or arginine decarboxylase activity. It is particularly valuable in differentiating closely related taxa such as Klebsiella and Enterobacter species (via ornithine decarboxylase) and in supporting standard identification schemes for Salmonella, Shigella, and other non-fermentative Gram‑negative bacteria. The test can be integrated into wider biochemical panels for organism profiling in clinical diagnostics, food microbiology, and environmental testing.

Features and Benefits

Features:

• High-Quality Standards: NutriSelect^® Plus powdered culture media are made from carefully selected raw materials, ensuring consistent quality.
• Wide Range of Applications: Suitable for various microbiological needs, including academic research, food and beverage testing, and pharmaceutical applications.
• Regulatory Compliance: Products meet industry standards and are compliant with ISO 9001 quality assurance.
• Innovative Formulations: Includes novel media types such as non-animal-derived alternatives, synthetically defined media, and chromogenic media.

Benefits:

• Convenience and Safety: Easy-to-use powdered format with minimal clumping and excellent solubility, even in warm or humid conditions.
• Sustainability: Reduced risks associated with fine dust and toxic substance inhalation, ensuring a safer work environment.
• Extended Shelf Life: Products have a prolonged shelf life of up to five years.
• High Reproducibility: Ensures consistent results across batches, exceeding regulatory demands.
• Comprehensive Portfolio: A vast selection of over 350 products tailored to meet diverse microbiological testing needs.

Preparation Note

Suspend 10.52 g of Decarboxylase Broth Base in 1000 ml of distilled water. Add 10 g of either L-Arginine (Cat. No. 11010), or L-Lysine (Cat. No. 62840), or L-Ornithine (Cat. No. 75470). Heat to dissolve the medium completely. When L-ornithine is added, readjust the pH. Dispense in 5 ml quantities in screwcapped tubes. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 10 minutes.

Other Notes

Ingredients (g/L)
Peptic digest of animal tissue, 5.00
Beef extract, 5.00
Dextrose, 0.50
Bromocresol purple, 0.01
Cresol red, 0.005
Pyridoxal, 0.005

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)