KK4752

KAPA PROBE FAST One-Step

Universal

• Synonym(s): RT-qPCR, probe qPCR, qPCR
• NACRES: NA.55
• UNSPSC Code: 41106300

Properties

• usage: sufficient for 500 reactions
• feature: dNTPs included: no, hotstart: no
• packaging: pkg of 500 RXNS
• manufacturer/tradename: Roche
• technique(s): RT-qPCR: suitable
• input: purified DNA
• detection method: probe-based
• storage temp.: −20°C

Description

General description

KAPA PROBE FAST One-Step qRT-PCR Master Mix (2X) Universal Kit is a sensitive and convenient solution for real-time PCR using RNA as template. The kit is designed for high-throughput, fast-cycling, one-step RNA quantification using sequence-specific fluorogenic probes. It is compatible with all fluorogenic probe-based technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g. TaqMan^®) and displacement probes (e.g., molecular beacons)

Application

KAPA PROBE FAST One-Step qRT-PCR Master Mix (2X) Universal has been used for:

• Gene expression analysis
• SNP/mutation analysis
• Gene knockdown validation
• RT-PCR

Features and Benefits

Quick Notes:

• The kit is suitable for all fluorogenic probe-based technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan) and displacement probes (e.g., molecular beacons).
• Use only gene-specific primers for one-step qRT-PCR.
• Optimal cDNA synthesis is achieved at 42°C for 5 min.
• 3 min at 95°C is sufficient for RT inactivation and DNA polymerase activation.
• Do not exceed 25 μL reaction volumes

Packaging

Kit includes qPCR Master Mix (2X), KAPA RT Mix (50X), dUTP (10 mM), ROX High Reference Dye (50X) and ROX Low Reference Dye (50X)

Preparation Note

Always ensure that components have been fully thawed and thoroughly mixed before use. The KAPA PROBE FAST qPCR Master Mix may not freeze solidly, even when stored at -20 °C. The KAPA RT Mix is temperature-sensitive, and should be stored at -20 °C and kept on ice during use. ROX reference dye is light-sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.

Analysis Note

All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Legal Information

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash