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TMKIT

mPAGE®TurboMix Bis-Tris Gel Casting Kit

Premixed solutions for casting bis-tris polyacrylamide gels; TMKIT-10 includes 36 mL resolving solution and 20 mL stacking solution; TMKIT-60 includes 216 mL resolving solution and 120 mL stacking solution

Synonym(s):

Bis-Tris gel casting kit, SDS-PAGE gel casting kit, gel casting kit, protein gel casting kit

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About This Item

NACRES:
NA.25
UNSPSC Code:
41105319
Pricing and availability is not currently available.
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Quality Level

200

sterility

0.2 μm filtered

form

liquid

feed ratio

29:1

storage condition

protect from light

storage temp.

2-8°C

General description

mPAGE® TurboMix Bis-Tris Gel Casting Kits deliver all the benefits of bis-tris gel chemistry. The gels operate at neutral pH which minimizes protein modification and promotes protein stability during the run. This leads to sharper bands and improved resolution. Run times are about 40% faster than standard tris-glycine gels. Cast gels can be stored at 4 °C for up to 4 weeks and are compatible with standard staining and transfer methods.
The mPAGE® TurboMix casting workflow offer many improvements over standard processes.

  • Pre-mixed acrylamide buffer solutions save time and improve reproducibility
  • Flexibility: dilute resolving solution with DI water to any gel percentage between 8% and 15%
  • Quick Cast protocol allows simultaneous polymerization of stacking and resolving gels, saving even more time

Application

Premixed solutions for casting bis-tris polyacrylamide gels.

Legal Information

MPAGE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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mPAGE®TurboMix Bis-Tris Gel Casting Kit Premixed solutions for casting bis-tris polyacrylamide gels; TMKIT-10 includes 36 mL resolving solution and 20 mL stacking solution; TMKIT-60 includes 216 mL resolving solution and 120 mL stacking solution

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mPAGE® TurboMix Stacking Solution 120ML Premixed stacking gel solution for casting bis-tris polyacrylamide gels

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mPAGE® Gel Casting Device Kit for hand casting mini gels with a 0.75 mm, 1.0 mm or 1.5 mm thickness, includes plates and combs

MGCK

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storage condition

protect from light

storage condition

-

storage condition

protect from light

storage condition

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

form

liquid

form

-

form

liquid

form

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

feed ratio

29:1

feed ratio

-

feed ratio

29:1

feed ratio

-

sterility

0.2 μm filtered

sterility

-

sterility

0.2 μm filtered

sterility

-

Certificates of Analysis (COA)

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Global Trade Item Number

SKUGTIN
TMKIT-6004065265094719
TMKIT-1004065265094702

Questions

1–3 of 3 Questions  

  1. In TMKIT mPAGE kit details 1. Flexibility: dilute resolving solution with DI water to any gel percentage between 8% and 15% Could you please confirm the stock resolving solution percentage? 2. Feed ratio 29:1 If the feed ratio indicates the ratio

    1 answer

    1. This kit may be used to prepare gels at a range of 8 - 15%. An 8% gel requires 2.4 mL Resolving Solution, a 10% requires 3 mL, a 12% requires 3.6 mL, and a 15% requires 4.5 mL. See the table below for additional details. The feed ratio refers to the amount of acrylamide monomer to crosslinker.

      Additional information may be accessed in the Product User Guide at the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/132/613/20508849w-ms.pdf

      Helpful?

  2. What is the composition of the resolving (TMXR-36ML) and stacking (TMXS-25ML) solutions that are apart of the kit TMKIT?

    1 answer

    1. The composition is considered propreietary.

      Helpful?

  3. Is SDS present in the resolving (TMXR-36ML) and stacking (TMXS-25ML) solutions that are apart of the kit?

    1 answer

    1. SDS is not present in the formulation.

      Helpful?

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