MABS277
Anti-monoglycylated Tubulin Antibody, clone TAP 952
clone TAP 952, from mouse
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
TAP 952, monoclonal
species reactivity
human, sheep, sea urchin, porcine, mouse, Drosophila, snail
technique(s)
dot blot: suitable
immunofluorescence: suitable
western blot: suitable
isotype
IgG1κ
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TUBA1A(7846)
General description
Several post-translational modifications of tubulin have been identified in eukaryotic cells. Glycylation is a poly-modification which occurs as lateral branching of glycine chains of variable length identified in axonemal tubulin of Paramecium cilia. This modification has been detected on tubulin and/or cilia/flagella of many unicellular and pluricellular organisms. The differential distribution of distinct polyglycylated tubulin isoforms between cytoplasmic and axonemal compartments in Paramecium indicates that the polyglycylation level of tubulin is highly regulated at the cell level. In Paramecium, the TAP 952 antibody is reactive on cytoplasmic and axonemal tubulin. In metazoan cells and/or cell lines, it is specific for tubulin of motile cilia and primary cilia, and thus useful for motile cilia and primary cilia detection.
Specificity
This antibody recognizes the C-terminus of monoglycylated alpha and beta-tubulins.
Can be used as a marker for motile cilia.
Can be used as a marker for motile cilia.
Immunogen
Electroeluted Paramecium axonemal tubulin
Epitope: Amino acids 427-449 of monoglycylated alpha-tubulin and 427-442 of monoglycylated beta-tubulin (Bré et al., 1998, Mol. Biol. Cell 9, 2655-2665)
Application
Detect Tubulin using this mouse monoclonal antibody, Anti-monoglycylated Tubulin Antibody, clone TAP 952 validated for use in western blotting, Immunofluorescence & Dot Blot.
Immunofluorescence Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in mouse spermatozoa (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).
Dot Blot Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in synthetic monoglycylated tubulin peptides (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).
Western Blot Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in a panel of total protein extracts from select species (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).
Immunoflourescence Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in lemur, human, and sea urchin spermatozoa (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).
Dot Blot Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in synthetic monoglycylated tubulin peptides (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).
Western Blot Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in a panel of total protein extracts from select species (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).
Immunoflourescence Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in lemur, human, and sea urchin spermatozoa (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
Quality
Evaluated by Western Blotting on Paramecium total cytoskeletal extract.
Western Blotting Analysis: A 1:50,000 dilution of this antibody detected monoglycylated Tubulin in 10 µg of Paramecium total cytoskeletal proteins.
Western Blotting Analysis: A 1:50,000 dilution of this antibody detected monoglycylated Tubulin in 10 µg of Paramecium total cytoskeletal proteins.
Target description
~50 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Katerina A Turner et al.
microPublication biology, 2022 (2022-11-30)
Sperm cells are transcriptionally and translationally silent. Therefore, they may use one of the remaining mechanisms to respond to stimuli in their environment, the post-translational modification of their proteins. Here we examined three post-translational modifications, acetylation, glutamylation, and glycylation of
Liliya Nazlamova et al.
Frontiers in genetics, 13, 1009430-1009430 (2022-10-01)
Retinitis pigmentosa (RP) is the most common cause of hereditary blindness, and may occur in isolation as a non-syndromic condition or alongside other features in a syndromic presentation. Biallelic or monoallelic mutations in one of eight genes encoding pre-mRNA splicing
Ying Hsu et al.
Human molecular genetics, 30(1), 87-102 (2021-02-01)
The BBSome is a protein complex consisting of BBS1, BBS2, BBS4, BBS5, BBS7, BBS8, BBS9 and BBS18 that associates with intraflagellar transport complexes and specializes in ciliary trafficking. In primary cilia, ciliary entry requires the fully assembled BBSome as well
Haibo Xie et al.
Journal of molecular cell biology, 14(7) (2022-08-19)
Meiosis is essential for evolution and genetic diversity in almost all sexual eukaryotic organisms. The mechanisms of meiotic recombination, such as synapsis, have been extensively investigated. However, it is still unclear whether signals from the cytoplasm or even from outside
Abdullah O Khan et al.
Haematologica, Online ahead of print (2020-12-18)
In specialised cells, the expression of specific tubulin isoforms and their subsequent post-translational modifications drive and coordinate unique morphologies and behaviours. The mechanisms by which β1-tubulin, the platelet and megakaryocyte (MK) lineage restricted tubulin isoform, drives platelet production and function
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