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MABN466

Sigma-Aldrich

Anti-Ankyrin-G Antibody, clone N106/36

clone N106/36, from mouse

Synonym(s):

Ankyrin-3, ANK-3, Ankyrin-G

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

N106/36, monoclonal

species reactivity

rat

technique(s)

immunohistochemistry: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

General description

Ankyrins are a family of spectrin-binding proteins that couple the spectrin/actin cytoskeletal network to the cytoplasmic domains of integral membrane proteins which include channels such as the anion exchanger, voltage-dependent sodium channel, and the Na/K ATPase, as well as neuronal cell adhesion molecules related to L1. In the nervous system, three different ankyrins are known to exist; ankyrinB, ankyrinG, and ankyrinR. All three ankyrins contain an N-terminal membrane binding domain, an internal domain capable of binding spectrin, and a C-terminal regulatory domain which is the target of alternative splicing. AnkyrinB, the predominant neuronal ankyrin includes two isoforms of ~220 kDa and ~440 kDa. The ~220 kDa alternatively spliced isoform is the major form present in the adult brain, while the ~440 kDa form is preferentially expressed in the developing neonatal brain. Three forms of ankyrinG (~190 kDa, ~270 kDa, ~480 kDa) are localized in axonal initial segments and the nodes of Ranvier of myelinated axons where fluxes of action potential occur and ion channels are enriched. Ankyrins comprise ~0.5%-1% of the total membrane protein in the adult vertebrate brain.

Specificity

Other homologies: Human (92% sequence homology), and Mouse (87% sequence homology).

Immunogen

Recombinant protein corresponding to human Ankyrin-G.

Application

Anti-Ankyrin-G Antibody, clone N106/36 is a highly specific mouse monoclonal antibody & that targets Ankyrin & has been tested in IHC.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Ankyrin-G in rat cerebral cortex tissue.

Immunohistochemistry Analysis: A representative lot detected Ankyrin-G in rat optic nerve tissue.

Immunofluorescence Analysis: A representative lot detected Ankyrin-G in adult rat cortex tissue.

Immunofluorescence Analysis: A representative lot from an independent laboratory detected Ankyrin-G in a rat model of TLE mEC layer II neurons (Hargus, N. J., et al. (2011). Neurobiol Dis. 41(2):361-376.)
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Immunohistochemistry in rat hippocampus tissue.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Ankyrin-G in rat hippocampus tissue.

Target description

480 kDa calculated

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2a κ in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat hippocampus tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yanxia Ding et al.
Neuroreport, 29(18), 1537-1543 (2018-10-16)
Recent studies have indicated that the structure of the axon initial segment (AIS) of neurons is highly plastic in response to changes in neuronal activity. Whether an age-related enhancement of neuronal responses in the visual cortex is coupled with plasticity
Anja Konietzny et al.
Journal of cell science, 137(8) (2024-03-25)
In neurons, the microtubule (MT) cytoskeleton forms the basis for long-distance protein transport from the cell body into and out of dendrites and axons. To maintain neuronal polarity, the axon initial segment (AIS) serves as a physical barrier, separating the
Zhuang Xu et al.
iScience, 27(3), 109264-109264 (2024-03-07)
The axon initial segment (AIS) is located at the proximal axon demarcating the boundary between axonal and somatodendritic compartments. The AIS facilitates the generation of action potentials and maintenance of neuronal polarity. In this study, we show that the location
Baptiste Libé-Philippot et al.
Cell, 186(26), 5766-5783 (2023-12-23)
The enhanced cognitive abilities characterizing the human species result from specialized features of neurons and circuits. Here, we report that the hominid-specific gene LRRC37B encodes a receptor expressed in human cortical pyramidal neurons (CPNs) and selectively localized to the axon
Shogo Togawa et al.
Behavioural brain research, 468, 115040-115040 (2024-05-10)
Neurotoxins have been extensively investigated, particularly in the field of neuroscience. They induce toxic damage, oxidative stress, and inflammation on neurons, triggering neuronal dysfunction and neurodegenerative diseases. Here we demonstrate the neuroprotective effect of a silicon (Si)-based hydrogen-producing agent (Si-based

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