8.52011

Fmoc-Leu-OH

Novabiochem^®

• Synonym(s): Fmoc-Leu-OH, N-α-Fmoc-L-leucine
• Empirical Formula (Hill Notation): C₂₁H₂₃NO₄
• CAS Number: 35661-60-0
• Molecular Weight: 353.41
• UNSPSC Code: 12352209
• EC Index Number: 252-662-7
• NACRES: NA.22
• MDL number: MFCD00037133

Properties

• Quality Level: 400
• product line: Novabiochem^®
• assay: ≥98% (TLC), ≥98.0% (acidimetric), ≥99.0% (HPLC)
• form: powder
• reaction suitability: reaction type: Fmoc solid-phase peptide synthesis
• manufacturer/tradename: Novabiochem^®
• mp: 145-153 °C
• application(s): peptide synthesis
• functional group: Fmoc
• storage temp.: 2-30°C
• SMILES string: N(C(CC(C)C)C(=O)O)C(=O)OCC1c2c(cccc2)c3c1cccc3
• InChI: 1S/C21H23NO4/c1-13(2)11-19(20(23)24)22-21(25)26-12-18-16-9-5-3-7-14(16)15-8-4-6-10-17(15)18/h3-10,13,18-19H,11-12H2,1-2H3,(H,22,25)(H,23,24)
• InChI key: CBPJQFCAFFNICX-UHFFFAOYSA-N

Description

General description

High purity Fmoc-protected amino acid for research and process production of peptides, with very low levels of dipeptide, free-amino acids and acetic acid impurities. Standard building block for introduction of leucine amino-acid residues by Fmoc SPPS

Associated Protocols and Technical Articles
Fmoc-amino acids for Peptide Production
Cleavage and Deprotection Protocols for Fmoc SPPS

Application

• Fmoc-protection efficiency: Addressing the efficiency and recrystallization needs in the Fmoc-protection process (R Yoshino et al., 2017).
• Isotopic labeling for NMR: Development of 17O isotopic labeling of N-Formyl-MLF-OH and related building blocks, enhancing NMR spectroscopy applications (M Ha et al., 2021).
• Perovskite solar cell modification: Investigating the role of Fmoc-Leu-OH in modifying perovskite film to enhance solar cell efficiency (J Song et al., 2022).

Analysis Note

Colour (visual): white to off white
Appearance of substance (visual): powder
Colour index (0,5 M in DMF): ≤ 150 Hazen
Identity (IR): passes test
Enantiomeric purity: ≥ 99.8 % (a/a)
Purity (HPLC): ≥ 99.0 % (a/a)
Fmoc-ß-Ala-OH (HPLC): ≤ 0.1 % (a/a)
Fmoc-ß-Ala-Leu-OH (HPLC): ≤ 0.1 % (a/a)
Fmoc-Leu-Leu-OH (HPLC): ≤ 0.1 % (a/a)
Fmoc-Ile-OH (GC): ≤ 0.1 % (a/a)
Assay free amino acid (GC): ≤ 0.2 %
Purity (TLC(011A)): ≥ 98 %
Purity (TLC(0811)): ≥ 98 %
Solubility (25 mmole in 50 ml DMF): clearly soluble
Assay (acidimetric): ≥ 98.0 %
Water (K. F.): ≤ 2.0 %
Ethyl acetate (HS-GC): ≤ 0.5 %
Acetate (IC): ≤ 0.02 %

To see the solvent systems used for TLC of Novabiochem^® products please click here.

Other Notes

Replaces: 04-12-1025

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3