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  • [Simultaneous determination of six antibiotics in disinfection products by high performance liquid chromatography tandem mass spectrometry].

[Simultaneous determination of six antibiotics in disinfection products by high performance liquid chromatography tandem mass spectrometry].

Wei sheng yan jiu = Journal of hygiene research (2012-05-23)
Hui Fu, Xiaojian Hu, Changming Ding, Shaobin Lin
RÉSUMÉ

To develop a method for determination of the Metronidazole, Chlortetracycline hydrochloride, Oxytetracycline dihydrate, Minocycline hydrochloride, Erythromycin and Tetracycline hydrochloride in disinfection products by high performance liquid chromatography tandem mass spectrometry(LC-MS/MS). Samples were extracted by methanol and filtered through 0.45 microm PTFE membrane filter, then analyzed by LC-MS/MS using Waters Symmetry C18 (2.1 mm x 150 mm, 3.5 microm) column in positive ion scan mode. The mobile phase was 5 mmol/L ammonium acetate, methanol and acetonitrile. The linear range was 0-2000 ng/ml and the correlation coefficients were more than 0.998, the average recoveries ranged from 74.7% to 114% with the relative standard deviations between 1.6%-20.2%. The method was successfully used to detect the content of antibiotics in 115 disinfection products. The method is simple, sensitive, selective and suitable for the analysis of residual content of antibiotics in cream formulations of disinfection products.

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Description du produit

Supelco
Chlortetracycline hydrochloride, VETRANAL®, analytical standard
Sigma-Aldrich
Chlortetracycline hydrochloride, ≥91.0% dry basis (HPLC)
Sigma-Aldrich
Chlortetracycline hydrochloride, suitable for fluorescence, BioReagent, from Streptomyces aureofaciens, ≥85.0% (HPLC)