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A Simple Method for Immunohistochemistry and Imaging of Mosquito Salivary Glands.

Cold Spring Harbor protocols (2022-08-13)
Ines Martin-Martin, Thiago Luiz Alves E Silva, Adeline E Williams, Joel Vega-Rodriguez, Eric Calvo
RÉSUMÉ

Immunohistochemistry is a valuable technique that provides information on protein localization and interactions in tissues. Mosquito salivary gland immunohistochemistry requires the meticulous dissection of a delicate tissue. The integrity of the salivary glands must be closely monitored throughout the entire process to prevent structural damage and loss of saliva. This protocol describes a series of simple steps to perform salivary gland immunohistochemistry including tissue dissection, permeabilization, immunostaining, mounting, and imaging by confocal microscopy.

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DAPI, for nucleic acid staining
Sigma-Aldrich
Albumine de sérum bovin solution, 35% in 0.85% sodium chloride, aseptically filled