G004433

HPLC Analysis of Chloramphenicol in Milk on Ascentis^® C18 after SPE using SupelMIP^® SPE-Chloramphenicol

application for SPE, application for LC-MS

Propriétés

• Technique(s): LC/MS: suitable; solid phase extraction (SPE): suitable
• Paramètres du test: sample preparation: SPE (Solid Phase Extraction); sample/matrix: whole pasteurized milk spiked with chloramphenicol at 15 ng/mL and 38 ng/mL; SPE tube/cartridge: SupelMIP SPE - Chloramphenicol, 25 mg/10mL (LRC) (53210-U); condition: 1 mL methanol;1 mL DI water; sample addition: 1 mL; washing: 2 x 1 mL MS-grade water, 1 mL 5% acetonitrile in 0.5% acetic acid, 2 x 1 mL MS-grade water, 1 mL 20% acetonitrile in 1% ammonium hydroxide. (dry SPE tubes for 15 min. under gentle vacuum; 3 x 1 mL dichloromethane; dry SPE tubes for 1 min. under gentle vacuum); elution: elute chloramphenicol with 2 x 1 mL methanol:acetic acid:MS-grade water (89:1:10, v/v/v); eluate post-treatment: evaporate combined eluate to dryness at 50 °C under nitrogen. reconstitute in 150 mL LC mobile phase prior to LC-MS analysis; column: Ascentis C18, 2.1 mm x 10 cm I.D., 3 μm particles (581301-U); mobile phase: [A] 100 mM ammonium acetate: [B] MS-grade water: [C] acetonitrile (10:60:30, A:B:C); flow rate: 0.2 mL/min; column temp.: 35 °C; detector: MS, ESI(-), 320-323 m/z range; injection: 5 μL
• Adéquation: application for LC-MS; application for SPE
• Application(s): food and beverages

Description

Informations légales

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

SupelMIP is a registered trademark of Sigma-Aldrich Co. LLC