Accéder au contenu
MilliporeSigma
Toutes les photos(1)

Principaux documents

Voir toute la documentation

Z0276

Sigma-Aldrich

Anti-phospho-ZAP-70 (pTyr315/pTyr319) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Numéro MDL:
MFCD07370447
Code UNSPSC :
12352203

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous glycerol solution

Espèces réactives

human, mouse

Technique(s)

western blot: 1:1,000 using Jurkat cells treated with H2O2.

Numéro d'accès UniProt

P43403

Conditions d'expédition

dry ice

Température de stockage

−20°C

Informations sur le gène

human ... ZAP70(7535)
mouse ... Zap70(22637)

Description générale

ZAP-70 is a protein tyrosine kinase that is expressed in natural killer cells and T-lymphocytes. ZAP-70 interacts with the ζ chain of the T cell antigen receptor (TCR) and subsequently modulates cell signaling. Phosphorylation of Tyr315 and Tyr319 residues regulates TCR-mediated signal transduction . Anti-phospho-ZAP-70 pTyr315/pTyr319) antibody is specific for human ZAP-70. The product is expected to react with mouse ZAP-70 (as its 100% homologous to human ZAP-70), but has not been tested for it.

Immunogène

synthetic phosphopeptide derived from the region of human ZAP-70 that contains tyrosines 315 and 319.

Application

Anti-phospho-ZAP-70 pTyr315/pTyr319) antibody is suitable for use in western blot (1:1,000 using Jurkat cells treated with H2O2 and immunoblot.

Forme physique

Solution in Dulbecco′s phosphate buffered saline (without Mg2+and Ca2+), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
SLBD9362V
054K0791

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

B L Williams et al.
The EMBO journal, 18(7), 1832-1844 (1999-04-15)
Accumulating evidence indicates that the interdomain B regions of ZAP-70 and Syk play pivotal roles in the coupling of T-cell antigen receptor (TCR) stimulation to the activation of downstream signaling pathways. The interdomain B region of ZAP-70 contains at least
V Di Bartolo et al.
The Journal of biological chemistry, 274(10), 6285-6294 (1999-02-26)
Following T cell antigen receptor (TCR) engagement, the protein tyrosine kinase (PTK) ZAP-70 is rapidly phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family PTK Lck. These events have been implicated in
A C Chan et al.
Cell, 71(4), 649-662 (1992-11-13)
Protein-tyrosine kinases (PTKs) play an integral role in T cell activation. Stimulation of the T cell antigen receptor (TCR) results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR zeta chain, which can mediate

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique