U4757

Anti-Urocortin antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Numéro MDL: MFCD01632996
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.46

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: IgG fraction of antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Espèces réactives: rat, human
• Technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using microwave-treated sections of human placental tissue; quantitative precipitin assay: 1:10,000 using ¹²⁵I-Tyr⁰-Urocortin (rat)
• Numéro d'accès UniProt: P55089
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... UCN(7349) ; rat ... Ucn(29151)

Description

Description générale

Urocortin (UCN) is a neuropeptide, widely distributed in the central nervous system (CNS). Urocortin is derived from a larger precursor protein (pro-urocortin, also named corticotensin).

Urocortin (UCN) is encoded by the gene mapped to human chromosome 2p21–23. The encoded protein belongs to the corticotropin-releasing hormone (CRH) family. UCN is a 40 amino acid long peptide. It is widely expressed in the heart, lung, skin and immune cells.

Spécificité

The antibody is evaluated for activity and specificity by radioimmunoassay (RIA) and immunohistochemistry. The antibody does not cross-react with human or rat CRF (6-33), sheep CRF, PACAP38, human ACTH, human ACTH (18-39), and sauvagine.

Immunogène

synthetic peptide corresponding to the C-terminal of human urocortin (amino acids 25-40 with N-terminally added lysine) conjugated to KLH. The immunogen sequence (human) corresponds to pro-urocortin (amino acids 105-120) and is identical in rat urocortin.

Application

Anti-Urocortin antibody produced in rabbit has been used in:

• radioimmunoassay (RIA)
• immunohistochemistry
• immunocytochemistry

Anti-urocortin antibody produced in rabbit has been used to evaluate the entry of urocortin peptide into the brain.

Actions biochimiques/physiologiques

Urocortin (UCN) plays a vital role in inhibiting transforming growth factor β1 (TGFβ1) oncogenic signaling pathway. The encoded protein is implicated in systemic and peripheral stress-response systems. Mutations in UCN gene might increase the risk of susceptibility to obesity.

Urocortin has biological actions like corticotropin-releasing factor (CRF) such as autonomic and behavioral responses in mammalian species. It is a potent stimulator of adrenocorticotropic hormone (ACTH) secretion by corticotroph cells from the anterior pituitary in vitro and in vivo. In the central nervous system (CNS), urocortin acts to produce potent anxiogenic-like and appetite suppressing effects and peripherally has hypotensive effects.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable