The pACD4 vector set (no selectable marker) is a set of four linearized vectors intended for use with the TargeTron Gene Knockout System (TA0100) when the use of a selectable marker is not desired. Each vector in the pACD4 vector set is similar to the pACD4K-C TargeTron vector with the main difference being that they do not have a kanamycin RAM marker to select for chromosomal insertion. The pACD4 vector set also contains either an A, C, G, or T at the δ + 1 position.This is to facilitate proper base pairing of group II intron precursor RNA for different target site designs to allow for more efficient precursor RNA splicing to form active TargeTron RNPs.
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