T7076

Monoclonal Anti-Thimet Oligopeptidase 1 antibody produced in mouse

1 mg/mL, clone IVD6, purified immunoglobulin, buffered aqueous solution

• Synonyme(s) : Anti-THOP1
• Numéro MDL: MFCD08705604
• Code UNSPSC : 12352203

Propriétés

• Source biologique: mouse
• Conjugué: unconjugated
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: IVD6, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~85 kDa
• Espèces réactives: human, monkey
• Concentration: 1 mg/mL
• Technique(s): microarray: suitable; western blot: 0.5-1.0 μg/mL using SY5Y cell extract.
• Isotype: IgG2b
• Numéro d'accès UniProt: P52888
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... THOP1(7064)

Description

Description générale

Monoclonal Anti-Thimet Oligopeptidase 1 (THOP1) (mouse IgG2b isotype) is derived from the hybridoma IVD6 produced by the fusion of mouse myeloma cells (SP2/O cells) and splenocytes from CF1mice immunized with a synthetic peptide. THOP1 protein is found in tissues with high levels of neuropeptides and hormones. THOP1 gene is mapped on the human chromosome at 19p13.3.

Spécificité

Monoclonal Anti-Thimet Oligopeptidase recognizes human and monkey THOP1, ~85 kDa.

Immunogène

synthetic peptide corresponding to amino acids 47-63 of human THOP1.

Application

Monoclonal Anti-Thimet Oligopeptidase 1 antibody produced in mouse may be used in:

• immunoblotting
• immunocytochemistry
• immunohistochemistry
• enzyme-linked immunosorbent assay (ELISA)

Actions biochimiques/physiologiques

Thimet Oligopeptidase 1 (THOP1) is a metalloendopeptidase found in the brains of Alzheimer patients. It can cleave a synthetic peptide encompassing the amino-terminus of amyloid β-peptide and generate amyloidogenic fragments from recombinant human amyloid precursor protein (APP). THOP1 is involved in degrading peptides that are released from the proteasome and therefore limits the extent of major histocompatibility complex (MHC) class I antigen presentation. It is involved in the processing of bioactive peptides. Overexpression of the gene THOP1 in cells reduced the presentation of antigenic peptides by class I MHC molecules. Prevention of THOP1 expression by small interfering RNA promoted class I antigenic peptide presentation.

Description de la cible

THOP1 is a metalloprotease that is able to cleave a synthetic peptide encompassing the amino-terminus of amyloid b-peptideand generate amyloidogenic fragments from recombinant human Amyloid Precursor Protein.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)