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SRP6484

Sigma-Aldrich

Carbonic Anhydrase 2/CA2 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonyme(s) :

CA-II, CAII, Car2

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About This Item

Code UNSPSC :
12352204
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in HEK 293 cells

Étiquette/Marqueur

6-His tagged (C-terminus)

Pureté

≥95% (SDS-PAGE)

Forme

lyophilized

Poids mol.

calculated mol wt 30 kDa
observed mol wt 30 kDa (DTT-reduced. Ser 2 is the predicted N-terminal .)

Conditionnement

pkg of 10 μg
pkg of 50 μg

Impuretés

<1 EU/μg endotoxin (LAL test)

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... CA2(760)

Description générale

Carbonic Anhydrase 2 (CA2) is a zinc metalloenzyme and it helps in regulating acid-base balance in cells. It is a ubiquitous cytosolic protein. Carbonic anhydrase 2 also known as carbonate dehydratase II, carbonic anhydrase C, belongs to the class of a carbonic anhydrases. The associated gene is mapped to human chromosome 8q21.

Application

Carbonic Anhydrase 2/CA2 human has been used for CA2 ELISA (enzyme-linked immunosorbent assay).

Actions biochimiques/physiologiques

Carbonic Anhydrase 2 (CA2) catalyzes the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa). CA2 can bind to various transporters, such as anion exchanger 1 (AE1), NBCe1 (Na(+)/HCO3(-) cotransporter), NHE3 (Na(+)/H(+) exchanger 3) and MCT (monocarboxylate transporter). This association increases the rate of transport. Mutations in the CA2 gene are responsible for carbonic anhydrase II (CAII) deficiency syndrome, an autosomal recessive osteopetrosis characterised with renal tubular acidosis as well as cerebral calcifications. Changes in the expression of CA2 are linked with carcinogenesis.

Forme physique

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, pH 8.0, with 150 mM NaCl, 1 mM DTT. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Eye Irrit. 2

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Metabolon disruption: a mechanism that regulates bicarbonate transport.
Alvarez BV, et al.
The Embo Journal, 24, 2499-2499 (2005)
Carbonic anhydrase II binds to and increases the activity of the epithelial sodium-proton exchanger, NHE3.
Krishnan D, et al.
American Journal of Physiology: Renal Physiology, 309, F383-F383 (2015)
A study of anti-carbonic anhydrase II antibodies in rheumatic autoimmune diseases.
Ono M, et al.
Journal of Dermatological Science, 21, 183-183 (1999)
Two novel CAII mutations causing carbonic anhydrase II deficiency syndrome in two unrelated Chinese families.
Pang Q, et al.
Metabolic Brain Disease, 30, 989-989 (2005)
Carbonic anhydrase II: a novel biomarker for pseudomyxoma peritonei.
Jarvinen P, et al.
Acta Pathologica, Microbiologica et Immunological Scandinavica, 125, 207-207 (2017)

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