SRP5116
PCAF (431-end), GST tagged human
recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonyme(s) :
CAF, GCN5, GCN5L, GCN5L1, P/CAF
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About This Item
Source biologique
human
Produit recombinant
expressed in E. coli
Essai
≥70% (SDS-PAGE)
Forme
buffered aqueous glycerol solution
Poids mol.
~69 kDa
Numéro d'accès NCBI
Application(s)
cell analysis
Conditions d'expédition
dry ice
Température de stockage
−70°C
Informations sur le gène
human ... KAT2B(8850)
Description générale
PCAF is a protein that shows homology to the yeast GCN5 and associates with p300/CBP. PCAF has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. PCAF encodes a predicted 832-amino acid protein that showed by RNA blotting to be expressed in all tissues, most strongly in heart and skeletal muscle. PCAF has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. PCAF is required for MyoD activity and muscle differentiation. PCAF directly acetylated MyoD which then displays an increased affinity for its DNA target.
p300/CBP-associated factor (PCAF) is a member of the histone acetyltransferases (HATs) protein family.[1] It is also known as KAT2B (lysine acetyltransferase 2B), and is the catalytic subunit of the megadalton metazoan complex ATAC (Ada-Two-A containing complex). In animals, this protein is composed of an N terminal extension region, a HAT domain and a bromodomain (BRD). An acetyl-CoA binding pocket is present in the HAT domain, which is globular in structure.[2] This protein is composed of over 2400 amino acids.[3]
Actions biochimiques/physiologiques
p300/CBP-associated factor (PCAF) functions as a co-activator of the tumor suppressor gene p53, and transactivates target genes along with p53, by acetylating both bound p53 and histones within p53 target promoters. Studies in breast cancer cells show that PCAF is a p53 target gene, and its induction results in increased p53 binding to PCAF promoter.[1] This protein interacts with multiple DNA-binding transcriptional factors such as nuclear hormone receptors, CREB (cAMP response element-binding, c-Jun/v-Jun, c-Myb/v-Myb, Sap-1a, c-Fos, and MyoD (myogenic differentiation).[3]
Forme physique
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Notes préparatoires
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Dimeric structure of p300/CBP associated factor.
Shi S, et al.
BMC Structural Biology, 14(1), 2-2 (2014)
V Sartorelli et al.
Molecular cell, 4(5), 725-734 (2000-01-05)
p300/CBP and PCAF coactivators have acetyltransferase activities and regulate transcription, cell cycle progression, and differentiation. They are both required for MyoD activity and muscle differentiation. Nevertheless, their roles must be different since the acetyltransferase activity of PCAF but not of
The transcriptional coactivators p300 and CBP are histone acetyltransferases.
Ogryzko VV, et al.
Cell, 87(5), 953-959 (1996)
The acetyltransferase p300/CBP-associated factor is a p53 target gene in breast tumor cells.
Watts GS, et al.
Neoplasia, 6(3), 187-194 (2004)
X J Yang et al.
Nature, 382(6589), 319-324 (1996-07-25)
The adenoviral oncoprotein E1A induces progression through the cell cycle by binding to the products of the p300/CBP and retinoblastoma gene families. A new cellular p300/CBP-associated factor (P/CAF) having intrinsic histone acetylase activity has been identified that competes with E1A.
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