SRP0339

Ceramide Kinase FLAG tag active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥40% (SDS-PAGE)

• Synonyme(s) : CERK, LK4, hCERK
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in baculovirus infected Sf9 cells
• Pureté: ≥40% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 60 kDa
• Conditionnement: pkg of 10 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Concentration: 0.4 mg/mL
• Numéro d'accès NCBI: NM_022766
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... CERK(64781)

Description

Description générale

Ceramide kinase (CERK) is a 537-amino acid protein. It has a catalytic region, a putative amino-myristoylation site in its amino terminal region and a pleckstrin homology domain. The gene encoding CERK is localized on human chromosome 22q13.31.

Human ceramide kinase (CERK) (GenBank Accession No. NM_022766), 1-537 (end) with C-terminal Flag-tag, MW= 60 kDa, expressed in Baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Actions biochimiques/physiologiques

Ceramide kinase (CERK) is an enzyme involved in sphingolipid metabolism and in the synthesis of ceramide-1-phosphate. It associates with calmodulin and at the time of Fc receptor-mediated phagocytosis, it aggregates in lipid rafts. CERK may have a role in calcium signaling. The protein functions in the phagocytosis of polymorphonuclear leukocytes and degranulation of mast cells.

Définition de l'unité

One unit is defined as the amount of enzyme required to produce 1 pmole of ceramide-1-phosphate/min at 37°C.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable