SRP0208

PRL-1 (2-173) Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Synonyme(s) : PRL-1, PTP4A1, Protein tyrosine phosphatase type IVA, member 1, Protein-tyrosine phosphatase of regenerating liver 1
• Code UNSPSC : 51111800
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Essai: ≥90% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 19.8 kDa
• Conditionnement: pkg of 50 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Concentration: >0.02 mg/mL
• Numéro d'accès NCBI: BC023975
• Numéro d'accès UniProt: Q93096
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... PTP4A1(7803)

Description

Description générale

Human PRL 1 (GenBank Accession No. BC023975), (amino acids 2-173) with N-terminal HIS tag, MW = 19.8 kDa, expressed in an E. coli expression system.

Protein-tyrosine-phosphatase of regenerating liver 1 (PRL1), also known as protein tyrosine phosphatase type IVA, member 1 (PTP4A1), is encoded by the gene mapped to human chromosome 6q12. The encoded protein is a member of a small class of prenylated protein tyrosine phosphatases (PTPs). It is characterized by a PTP domain and a specific C-terminal prenylation motif. PRL1 is mainly localized to the cell membrane and the endoplasmic reticulum depending on its C-terminal prenylation.[1][2]

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Actions biochimiques/physiologiques

Protein-tyrosine-phosphatase of regenerating liver 1 (PRL1) or protein tyrosine phosphatase type IVA, member 1 (PTP4A1) regulates cancer-related signaling pathways, which are downstream of epidermal growth factor receptor (EGFR) pathway and stimulates cell proliferation, invasion and migration. PRL1 acts as a potential therapeutic target and predictor for patient survival in prostate cancer (PCa) including those with castration-resistant prostate cancer (CRPC). PRL1 is essential for normal mitotic process. Elevated expressions of prenylated protein tyrosine phosphatases (PTPs) in mammalian cells conferred a transformed phenotype, which might be involved in the tumorigenesis.[2][3]

Définition de l'unité

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.