SRP0185

ALAS2 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Synonyme(s) : ALA synthase 2-δ, ALAS-E, Aminolevulinic acid synthase 2
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Pureté: ≥90% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 46 kDa
• Conditionnement: pkg of 100 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Concentration: >0.02 mg/mL
• Numéro d'accès NCBI: NM_000032
• Numéro d'accès UniProt: P22557
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... ALAS2(212)

Description

Description générale

Human ALAS2 (aminolevulinate, δ-, synthase 2) (GenBank Accession No. NM_000032), (amino acids 136-553) with N-terminal His tag, MW = 46 kDa, expressed in an E. coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol, 3 mM DTT, 20 μM pyridoxal 5′ phosphate monohydrate.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.