SRP0134

EZH2/EED/SUZ12/RbAp48/AEBP2 human

recombinant, expressed in baculovirus infected insect cells

• Synonyme(s) : ENX-1, EZH2, Enhancer of zeste homolog 2, KMT6A, Lysine N-methyltransferase 6A
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in baculovirus infected insect cells
• Pureté: ≥80% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 48 kDa (RbAp48); 51 kDa (EED); 53 kDa (AEBP2); 86 kDa (EZH2); 87 kDa (SUZ12)
• Conditionnement: pkg of 50 μg
• Concentration: >0.02 mg/mL
• Numéro d'accès NCBI: NM_003797 (EED); NM_004456 (EZH2); NM_005610 (RbAP48); NM_015355 (SUZ12); NM_153207 (AEBP2)
• Numéro d'accès UniProt: Q15910
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... EZH2(2146)

Description

Description générale

EZH2, EED, SUZ12, RbAp48 and AEBP2 are components of the polycomb repressive complex 2 (PRC2). The highly conserved complex methylates lysine 27 of histone H3. It also has a role in gene silencing and transcription repression.

Application

EZH2/EED/SUZ12/RbAp48/AEBP2 human is useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling.

Définition de l'unité

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable