SAB4200649

Monoclonal Anti-MASTL antibody produced in mouse

clone 2C8/D11, purified from hybridoma cell culture

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 2C8/D11, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: ~100 kDa
• Espèces réactives: human, monkey
• Concentration: ~1 mg/mL
• Technique(s): flow cytometry: 10-20 μg/test using using human HeLa cells.; immunoblotting: 1-2 μg/mL using whole extracts of human HeLa cells.; immunofluorescence: 10-20 μg/mL using using human HeLa cells.
• Isotype: IgG2b
• Numéro d'accès UniProt: Q96GX5
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Mastl(67121)

Description

Description générale

Monoclonal Anti-MASTL (mouse IgG2b isotype) is derived from the hybridoma 2C8/D11 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein. Microtubule-Associated Serine/Threonine kinase-Like (MASTL), also known as GREATWALL (GWL) in Drosophila and Xenopus is localized to the nucleus but, during M-phase it is activated and re-localized to the centrosomes.

Immunogène

immunized with a fusion protein of 250aa polypeptide from the region that divides the kinase domain of mouse MASTL

Application

Monoclonal Anti-MASTL antibody produced in mouse may be used in various immunochemical techniques including immunoblotting, flow cytometry and immunofluorescence.

Actions biochimiques/physiologiques

Microtubule-associated serine/Threonine kinase-Like (MASTL) acts as a cell cycle marker of M phase. It has an ability to regulate mitosis entry, anaphase and cytokinesis. In Drosophila MASTL (Greatwall) was initially identified as a kinase essential for chromosome condensation. MASTL enhances cyclin B1-Cdk1-dependent mitotic phosphorylation events. Furthermore, MASTL is also an inhibitor of the tumor suppressor protein Phosphatase 2A (PP2A), and thus maintains the equilibrium between cyclin B-Cdk1 and PP2A. A missense mutation in the MASTL gene leads to the development of autosomal dominant inherited thrombocytopenia.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable