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SAB4200380

Sigma-Aldrich

Anti-LAYN antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonyme(s) :

Anti-LAYN antibody produced in rabbit, Anti-Layilin

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~43 kDa

Espèces réactives

human

Concentration

~1.0 mg/mL

Technique(s)

indirect immunofluorescence: 2.5-5.0 μg/mL using human A549 cells.
western blot: 1-2 μg/mL using whole extracts of HEK-293T cells overexpressing human LAYN.

Numéro d'accès UniProt

Q6UX15

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... LAYN(143903)

Description générale

Layilin (LAYN) is a cell surface hyaluronan receptor, originally identified as a binding partner of talin located in membrane ruffles of spreading cells. LAYN is widely expressed in different cell lines and tissues. This gene is mapped to human chromosome 11q.

Immunogène

synthetic peptide corresponding to an internal region of human LAYN, conjugated to KLH. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Application

Anti-LAYN antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Actions biochimiques/physiologiques

Layilin (LAYN) binds to hyaluronan, a ubiquitous extracellular matrix component involved in the invasion of a variety of tumour cells, through its extracellular domain and to cytoskeleton-membrane linker proteins including Merlin and Radixin, through its cytoplasmic domain, implicating LAYN in cell adhesion and motility. LAYN is overexpressed in the human lung carcinoma A549. LAYN down-regulation by RNA interference, inhibits invasion and lymphatic metastasis of A549 cells.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Down-regulation of layilin, a novel hyaluronan receptor, via RNA interference, inhibits invasion and lymphatic metastasis of human lung A549 cells
Chen Z, et al.
Biotechnology and Applied Biochemistry, 50(Pt), 2-2 (2008)
Layilin, a cell surface hyaluronan receptor, interacts with merlin and radixin
Bono, et al.
Experimental Cell Research, 308(1) (2005)
Stefan Deneberg et al.
Epigenetics, 9(6), 910-917 (2014-04-02)
A commonly deleted region in chronic lymphocytic leukemia (CLL) is the 11q22-23 region, which encompasses the ATM gene. Evidence suggests that tumor suppressor genes other than ATM are likely to be involved in CLL with del(11q). A microRNA (miR) cluster

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