SAB4200340

Anti-Golph3 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonyme(s) : Anti-GOPP1, Anti-GPP34, Anti-Golgi peripheral membrane protein 1, 34 kDa, Anti-Golgi phosphoprotein 3 (coat-protein), Anti-Golgi-associated protein, Anti-MIDAS, Anti-Mitochondrial DNA absence factor
• Code UNSPSC : 12352203

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~34 kDa
• Espèces réactives: human
• Concentration: ~1.0 mg/mL
• Technique(s): immunoprecipitation (IP): 5-10 μg using lysates of human HeLa cells.; western blot: 0.1-0.2 μg/mL using whole extracts of HEK-293 cells over-expressing human Golph3.
• Numéro d'accès UniProt: Q9H4A6
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... GOLPH3(64083) ; mouse ... Golph3(66629)

Description

Description générale

Golgi phosphoprotein 3 (Golph3) is a peripheral membrane protein of the Golgi stack that localizes to the trans-Golgi network. It is conserved from yeast to human. The GOLPH3 gene is mapped to the human chromosome 5p13.3 location.

Spécificité

Anti-Golph3 (C-terminal) recognizes human Golph3.

Immunogène

peptide corresponding to the C-terminal region of human Golph3, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey and canine.

Application

Anti-Golph3 (C-terminal) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Actions biochimiques/physiologiques

Golgi phosphoprotein 3 (Golph3) is a phosphatidylinositol-4-phosphate (PtdIns(4)P) binding protein required for Golgi function. It plays a vital role in Golgi trafficking and morphology by interacting with the unconventional myosin 18A (MYO18A) linking Golgi membranes to the F-actin cytoskeleton. GOLPH3 is an oncogene that is commonly targeted for amplification in human cancer and cancer cell lines. Furthermore, Golph3 overexpression is correlated with the hyperactivation of the mammalian target of rapamycin (mTOR) signaling in human cells.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable