SAB4200337

Anti-phospho-RasGRP1(pThr¹⁸⁴) antibody, Mouse monoclonal

clone p-RasGRP1-1, purified from hybridoma cell culture

• Synonyme(s) : Anti-CALDAG-GEFI, Anti-CALDAG-GEFII, Anti-RAS guanyl releasing protein 1 (calcium and DAG-regulated) hRasGRP1
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Conjugué: unconjugated
• Forme d'anticorps: purified from hybridoma cell culture
• Type de produit anticorps: primary antibodies
• Clone: p-RasGRP1-1, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~90 kDa
• Espèces réactives: human
• Concentration: ~1.0 mg/mL
• Technique(s): immunoprecipitation (IP): suitable; western blot: 2-4 μg/mL using whole extracts of HEK-293T cells overexpressing human RasGRP1
• Isotype: IgG2a
• Numéro d'accès UniProt: O95267
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: phosphorylation (pThr184)
• Informations sur le gène: human ... RASGRP1(10125) ; mouse ... Rasgrp1(19419) ; rat ... Rasgrp1(29434)

Description

Description générale

Monoclonal Anti-phospho-RasGRP1(pThr184) (mouse IgG2a isotype) is derived from the hybridoma p-RasGRP1-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic phospho-peptide corresponding to residues surrounding Thr184 of human RasGRP1 conjugated to KLH. RAS guanyl releasing protein 1 (RasGRP1) is a member of RasGRP family, characterized by the presence of a N-terminal Ras superfamily guanine nucleotide exchange factor (GEF) domain and and C-terminal calcium- and diacylglycerol (DAG)/phorbol ester-binding domains. It is an intracellular signaling protein. RasGRP1 has a N terminal guanine nucleotide exchange factor domain for Ras-like guanosine triphosphatases (GTPases) (RasGEFN/RasGEF), two EF hand motifs and a carboxy-terminal C1 domain.

Immunogène

a synthetic phospho-peptide corresponding to residues surrounding Thr¹⁸⁴ of human RasGRP1, conjugated to KLH. The corresponding sequence is identical in rat, mouse, monkey, bovine and canine RasGRP1.

Application

Monoclonal Anti-phospho-RasGRP1(pThr184) antibody produced in mouse has been used in immunoblotting and immunoprecipitation.

Actions biochimiques/physiologiques

RAS guanyl releasing protein 1 (RasGRP1) functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound guanosine diphosphate (GDP) for guanosine triphosphate (GTP). It activates the extracellular signal-regulated kinase (Erk)/mitogen-activated protein (MAP) kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable