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SAB4200170

Anti-CPSF4 antibody produced in rabbit

Name: Anti-CPSF4 antibody produced in rabbit
Brand: SIGMA

~1.0 mg/mL, affinity isolated antibody

Synonyme(s) :

Anti-CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR 4, Anti-CPSF30NS1 EFFECTOR DOMAIN-BINDING PROTEIN 1, Anti-NEB1

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About This Item

Code UNSPSC :

12352203

Nomenclature NACRES :

NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~30 kDa

Espèces réactives

human

Conditionnement

antibody small pack of 25 μL

Concentration

~1.0 mg/mL

Technique(s)

western blot: 1-2 μg/mL using Whole extracts of HEK-293T cells overexpressing human CPSF4.

Numéro d'accès UniProt

O95639

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CPSF4(10898)
mouse ... CPSF4(54188)
rat ... CPSF4(304277)

Description générale

Cleavage polyadenylation specificity factor 4 (CPSF4), also called CPSF 30 is a component of the CPSF complex. It comprises of five CCCH zinc finger motifs (ZF1-ZF5). CPSF4 gene is mapped to human chromosome 7q22.1.

Spécificité

Anti-CPSF4 recognizes human CPSF4.

Application

Anti-CPSF4 antibody produced in rabbit has been used in chromatin immunoprecipitation and immunoblotting.

Actions biochimiques/physiologiques

Cleavage polyadenylation specificity factor 4 (CPSF4) is a component of 3′-end mRNA processing machinery and binds to poly(U) sequence of RNA via the zinc finger motifs. These motifs are also involved in protein-protein interactions. CPSF4 along with other proteins primarily coordinate both cleavage and polyadenylation. Their interaction with the non-structural protein 1 (NS1) protein of the influenza virus leads to inhibition of polyadenylation event and 3′ end cleavage of pre-mRNA associated with the host. Elevated expression of CPSF4 is observed in lung adenocarcinomas.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Upregulation of cleavage and polyadenylation specific factor 4 in lung adenocarcinoma and its critical role for cancer cell survival and proliferation

Chen W, et al.

PLoS ONE, 8(12), e82728-e82728 (2013)

Identifying the molecular signature of the interstitial deletion 7q subgroup of uterine leiomyomata using a paired analysis.

Jennelle C Hodge et al.

Genes, chromosomes & cancer, 48(10), 865-885 (2009-07-16)

Uterine leiomyomata (UL), the most common neoplasm in reproductive-age women, have recurrent cytogenetic abnormalities including interstitial deletion of 7q. To develop a molecular signature, matched del(7q) and non-del(7q) tumors identified by FISH or karyotyping from 11 women were profiled with

3' end formation of pre-mRNA and phosphorylation of Ser2 on the RNA polymerase II CTD are reciprocally coupled in human cells.

Lee Davidson et al.

Genes & development, 28(4), 342-356 (2014-01-31)

3' end formation of pre-mRNAs is coupled to their transcription via the C-terminal domain (CTD) of RNA polymerase II (Pol II). Nearly all protein-coding transcripts are matured by cleavage and polyadenylation (CPA), which is frequently misregulated in disease. Understanding how

3? end formation of pre-mRNA and phosphorylation of Ser2 on the RNA polymerase II CTD are reciprocally coupled in human cells

Davidson L, et al.

Genes & Development, 28(4), 342-356 (2014)

Protein factors in pre-mRNA 3'-end processing.

C R Mandel et al.

Cellular and molecular life sciences : CMLS, 65(7-8), 1099-1122 (2007-12-26)

Most eukaryotic mRNA precursors (premRNAs) must undergo extensive processing, including cleavage and polyadenylation at the 3'-end. Processing at the 3'-end is controlled by sequence elements in the pre-mRNA (cis elements) as well as protein factors. Despite the seeming biochemical simplicity

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