P1870

Anti-p120^ctn antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonyme(s) : Anti-p120^cas
• Numéro MDL: MFCD00284066
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: IgG fraction of antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen 120 kDa
• Espèces réactives: human
• Technique(s): indirect immunofluorescence: 1:500 using human epidermoid carcinoma A431 cell line.; microarray: suitable; western blot: 1:2,000 using rat brain extract and human endothelial ECV304 whole cell extract.
• Numéro d'accès UniProt: O60716
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CTNND1(1500)

Description

Description générale

Catenin delta 1 (CTNND1), the 120 kDa catenin-related protein is also referred as catenin p120ctn, p120cas and pp120 src substrate. p120ctn contains a central armadillo repeat domain (ARM domain) and is structurally similar to β-catenin and γ-catenin. It codes for a member of the Armadillo (Arm) family of proteins. This gene is located on human chromosome 11q12.

Spécificité

The sequence is identical in mouse p120^ctn and isoforms 1, 2, 3, and 4 of human p120^ctn.

Immunogène

synthetic peptide corresponding to the sequence located near the C-terminus of human p120^ctn (catenin-related, isoform 1B, amino acids 881-900) conjugated to KLH.

Application

Anti-p120ctn antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Actions biochimiques/physiologiques

Catenin delta 1 (CTNND1/p120ctn) is described as a prominent substrate for the Src oncoprotein and a variety of receptor tyrosine kinases (RTKs) including those for epidermal growth factor (EGF), platelet derived growth factor (PDGF) and colony stimulating factor-1 (CSF-1). It can bind directly to the cytoplasmic domain of the epithelial E-cadherin via the armadillo repeat domain (ARM) domain. It co-precipitates in multiprotein complexes containing E-cadherin or other cadherins such as neural (N)- or placental (P)- cadherins, depending on the cell type. CTNND1 participates in the oncogenesis of some cancer. It can serve as a tumor oncogene in hepatocellular carcinoma (HCC).

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.