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MITOISO1

Sigma-Aldrich

Mitochondria Isolation Kit

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

Synonyme(s) :

Mitochondria Purification Kit

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About This Item

Numéro CE :
232-650-8
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Niveau de qualité

200

Utilisation

sufficient for 10-20 g (animal tissue)
 sufficient for 50 assays (2 mL)

Durée de conservation

2 yr

Technique(s)

fractionation: suitable

Conditions d'expédition

dry ice

Température de stockage

−20°C

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Catégories apparentées

Application

Mitochondria Isolation Kit has been used:
  • for the separation of mitochondrial and cytosolic fraction for determining the branched-chain aminotransferase (BCAT) activity
  • for the isolation of mitochondria from glomeruli and heart
  • for the isolation of mitochondria from injured brain samples for the measurement of mitochondrial transmembrane potential

The Mitochondria Isolation Kit provides a fast and easy isolation of an enriched mitochondrial fraction from animal tissues as well as the testing of the electrochemical proton gradient (ΔΨ) of the inner mitochondrial membrane. Useful for mitochondria mediated apoptosis studies.

Caractéristiques et avantages

  • Specially formulated extraction reagents & proven procedure suitable for small scale isolation - Isolate an enriched, intact mitochodrial fraction in a microfuge tube.
  • Produces functionally active, intact mitochondria - Resulting mitochondria are suitable for in vitro assays for apoptosis, oxidative stress or other studies.
  • Includes stain & protocol for determining inner membrane integrity - Determine the integrity of the inner mitochondrial membrane without the need to purchase other reagents.
  • Compatible with the Cytochrome c Oxidase Assay Kit - Allows easy determination of the integrity of the outer mitochondrial membrane

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • T9201Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solidFDS

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

CYTOCOX1

Cytochrome c Oxidase Assay Kit, sufficient for 100 tests, soluble and membrane bound mitochondria

CS0720

Citrate Synthase Assay Kit, 1 kit sufficient for 100 reactions (using a 1 ml cuvette), 1 kit sufficient for 480 reactions (using 96 multiwell plates)

MITOISO2

Mitochondria Isolation Kit, sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

CS0760

Isolated Mitochondria Staining Kit, 1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

MITOISO3

Yeast Mitochondria Isolation Kit, sufficient for 40 applications (using 20 OD culture preparations), isolation of an enriched mitochondrial fraction from yeast cells

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H315,H319,H334,H335

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Young-Kyoung Shin et al.
Cancer research, 65(8), 3162-3170 (2005-04-19)
5-Fluorouracil (5-FU) is widely used for treatment of advanced colorectal cancer. However, it is common for such patients to develop resistance to 5-FU, and this drug resistance becomes a critical problem for chemotherapy. The mechanisms underlying this resistance are largely
Mitochondrial dysfunction mediates aldosterone-induced podocyte damage: a therapeutic target of PPARgamma
Zhu C, et al.
The American Journal of Pathology, 178(5), 2020-2031 (2011)
Neuroprotective effect of hyperbaric oxygen therapy in brain injury is mediated by preservation of mitochondrial membrane properties
Palzur E, et al.
Brain Research, 1221, 126-133 (2008)
Jacinta Serpa et al.
The Journal of biological chemistry, 285(50), 39211-39223 (2010-10-12)
The short chain fatty acid (SCFA) butyrate is a product of colonic fermentation of dietary fibers. It is the main source of energy for normal colonocytes, but cannot be metabolized by most tumor cells. Butyrate also functions as a histone
Takehiko Dohi et al.
The Journal of clinical investigation, 114(8), 1117-1127 (2004-10-19)
Evasion of apoptosis is a hallmark of cancer, but the molecular circuitries of this process are not understood. Here we show that survivin, a member of the inhibitor of apoptosis gene family that is overexpressed in cancer, exists in a
Afficher tous les articles scientifiques apparentés

Articles

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Mitochondrial Stress and ROS

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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