The protocol is published in the product datasheet, available in the 'DOCUMENTATION' section of this page, under the 'Mor Documents' tab. Please see the link below to review:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/475/mini26bul.pdf
MINI26
PKH26 Red Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling
Distributed for Phanos Technologies
Synonyme(s) :
Red PKH membrane label kit
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| À vous/Référence | Disponibilité | Prix |
|---|---|---|
1 kit | Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit. | 470,00 $ |
A propos de cet article
NACRES:
NA.32
UNSPSC Code:
12352207
470,00 $
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
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pkg of 1 kit
Quality Segment
manufacturer/tradename
Distributed for Phanos Technologies
storage condition
protect from light
technique(s)
flow cytometry: suitable
fluorescence
λex 551 nm; λem 567 nm (PKH26 dye)
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
ambient
storage temp.
room temp
General description
This kit provides enough dye for up to 25 samples of 20 million cells each and enough diluent for up to 5 samples of 20 million cells each, when using a 2 mL final staining volume containing a final concentration of 2 x 10-6 M of PKH26 dye. Users must determine the optimal dye and cell concentrations from their cell type(s) and experimental purposes.
Application
PKH26 red fluorescent cell linker mini kit has been used for:
- labelling microglial, natural killer cells and preadipocytes
- tracking of viable transplanted adipose-derived stem cells
- in vitro proliferation studies of bone marrow stem cells
- general cell membrane labeling, including for in vitro cell labeling and long term in vivo cell tracking.
Other Notes
For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.
Legal Information
CellVue is a registered trademark of Phanos Technologies
1 of 1
Cet article | |||
|---|---|---|---|
| technique(s) flow cytometry: suitable | technique(s) flow cytometry: suitable | technique(s) - | technique(s) flow cytometry: suitable |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
| fluorescence λex 551 nm; λem 567 nm (PKH26 dye) | fluorescence λex 551 nm; λem 567 nm (PKH26 dye) | fluorescence λex 551 nm; λem 567 nm (PKH26 dye) | fluorescence λex 490 nm; λem 502 nm (PKH67 dye) |
| storage condition protect from light | storage condition protect from light | storage condition protect from light | storage condition protect from light |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| manufacturer/tradename Distributed for Phanos Technologies | manufacturer/tradename Distributed for Phanos Technologies | manufacturer/tradename Distributed for Phanos Technologies | manufacturer/tradename Distributed for Phanos Technologies |
Composants de kit seuls
Réf. du produit
Description
- Diluent C 10 mL
- PKH26 Cell Linker in ethanol .1 mL
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
Classe de stockage
3 - Flammable liquids
flash_point_f
57.2 °F - closed cup
flash_point_c
14 °C - closed cup
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MINI26-1KTの実験プロトコルを入手したいのですが、どこにありますか？
1 answer-
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Is it possible to lable cells with PKH26 at 4oC?
1 answer-
The membrane dyes will remain stable during the monoclonal staining at 4 deg C; however, capping of the monoclonal antibodies is highly probable if the general cell membrane labeling is carried out at ambient temperature subsequent to antibody labeling. As additional information may be required to address the question completely, please navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquiries" under the Products Section with all the information needed so that a member of the Technical Service team can reach out to assist further.
For more information, please refer to the product data-sheet below: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/475/mini26bul.pdf.
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