MAK468
NAD/NADH Assay Kit
sufficient for 100 colorimetric tests
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Produits recommandés
Niveau de qualité
Méthode de détection
colorimetric
Maladie(s) pertinente(s)
cancer
Conditions d'expédition
wet ice
Température de stockage
−20°C
Description générale
Pyridine nucleotides play an important role in metabolism, so there is interest in monitoring their concentration levels in metabolic systems. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and the redox state of cells or tissue. Nicotinamide adenine dinucleotide (NAD) is an enzymatic cofactor that plays a pivotal role in numerous redox reactions. Serving as an electron carrier, NAD seamlessly transitions between its oxidized form (NAD) and reduced form (NADH). Apart from its involvement in redox reactions, NAD assumes essential roles in ADP-ribosylation reactions and serves as a substrate for sirtuins.
Caractéristiques et avantages
- The linear detection range of the kit is 0.05 - 10 micromolar (µM).
- Compatible with high-throughput handling systems.
- Simple procedure involves adding a single working reagent, and reading the optical density at time zero and 15 minutes at room temperature.
Adéquation
The kit is suitable for NAD+/NADH concentration and ratio determination in cell or tissue extracts.
Principe
The NAD/NADH Assay Kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH with minimal interference (<1%) by NADP+/NADPH. The assay is a convenient method to measure NAD, NADH, and their ratio.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Met. Corr. 1
Code de la classe de stockage
8A - Combustible corrosive hazardous materials
Classe de danger pour l'eau (WGK)
WGK 2
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