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MAK228

Sigma-Aldrich

Calpain Activity Fluorometric Assay Kit

sufficient for 100 fluorometric tests

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About This Item

Code UNSPSC :
12352200

Méthode de détection

fluorometric

Maladie(s) pertinente(s)

endocrinological disorders, diabetes; neurological disorders; cancer; cardiovascular diseases

Température de stockage

−70°C

Description générale

Activation of calpain is involved in many forms of physiological and pathological processes (e.g., apoptosis). Calpain activation requires cell membrane and Ca2+, and activated calpain is released into cytosol. The Calpain Activity Assay Kit provides optimized buffers and reagents for a convenient measurement of calpain activity.

Adéquation

Suitable for analyzing calpain activity in apoptotic and other samples.

Principe

Calpain Activity Fluorometric Assay Kit is based on the cleavage of the calpain substrate, Ac-LLY-AFC. The Ac-LLY-AFC substrate emits blue light (max = 400 nm); upon cleavage by calpain, free AFC emits a yellow-green fluorescence ( max = 505 nm), which can be quantified using a fluorometer or a fluorescence plate reader. Comparison of the fluorescence intensity from a treated sample with a normal control allows determination of the changes in calpain activity.

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Classification des risques

Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids


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