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MAK055

Sigma-Aldrich

Kit d′analyse de l′activité de l′AST

sufficient for 100 colorimetric tests

Synonyme(s) :

Kit d’analyse de l’activité de la SGOT, Kit d’analyse de l’activité de la glutamo-oxaloacétique transaminase, Kit d’analyse de l’activité de la sérum glutamo-oxaloacétique transaminase, Kit d’analyse de l’activité de l’aspartate aminotransférase

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Le produit MAK055 n'est pas actuellement commercialisé dans votre pays Contacter notre Service technique

Utilisation

sufficient for 100 colorimetric tests

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

gastrointestinal diseases; cardiovascular diseases

Température de stockage

−20°C

Informations sur le gène

human ... GOT1(2805)
mouse ... GOT1(14718)
rat ... GOT1(24401)

Description générale

Le nouveau kit de dosage de l'AST (réf. MAK467) est désormais disponible ! L'aspartate aminotransférase (AST), anciennement appelée sérum glutamo-oxaloacétique transaminase (SGOT ou GOT), est une enzyme phosphate de pyridoxal (PLP)-dépendante qui catalyse la conversion de l'aspartate et du α-cétoglutarate en oxaloacétate et en glutamate. Comme c'est le cas pour l'alanine aminotransférase (ALT), les taux sanguins d'AST servent fréquemment de marqueur de la fonction hépatique. Cependant la distribution tissulaire de l'AST est plus large que celle de l'ALT, et les taux d'AST peuvent être perturbés en réponse à une maladie ou à des blessures au niveau de divers tissus, notamment les tissus squelettiques et cardiaques.

Application

Le Kit d′analyse de l′activité de l′AST est utilisé pour déterminer l′activité de l′aspartate aminotransférase dans les échantillons.[1][2]

Caractéristiques et avantages

Compatible avec les systèmes de manipulation d′échantillons à haut débit.

Adéquation

Convient à la mesure de l′activité de l′aspartate aminotransferase (AST) dans les échantillons biologiques, notamment les tissus, les cellules et le sérum.

Principe

Le Kit d′analyse de l′activité de l′aspartate aminotransférase propose une méthode de mesure simple et directe de l′activité de l′AST dans divers types d′échantillons. Dans ce kit, le transfert d′un groupement amino d′un aspartate à un α-cétoglutarate provoque la formation de glutamate, ce qui produit une coloration (450 nm) proportionnelle à l′activité enzymatique de l′AST présente. Une unité d′AST correspond à la quantité d′enzyme permettant de générer 1,0 μmole de glutamate par minute à pH 8,0 et 37 °C.

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Réf. du produit
Description
Tarif

Pictogrammes

Health hazardCorrosion
GHS08,GHS05

Mention d'avertissement

Danger

Mentions de danger

H314,H334

Classification des risques

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
8A06K07530
8M08K07530
8D13K07530
8G15K07530
8H22K07530

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Zhiqiang Ye et al.
Journal of cellular biochemistry, 119(9), 7397-7405 (2018-05-19)
Liver ischemia/reperfusion (I/R) injury has high mortality due to the intense inflammatory process occurs in the liver. However, the pathological mechanism underlying I/R injury is still not clear. Recent works showed that circular RNAs play critical roles in many human
Resolvin D1 and E1 alleviate the progress of hepatitis toward liver cancer in long-term concanavalin A-induced mice through inhibition of NF-KB activity.
Kuang H, et al.
Oncology Reports, 35, 307-317 (2016)
Leola N Chow et al.
PloS one, 11(3), e0151765-e0151765 (2016-03-22)
Modulation of chemokine CXCL12 and its receptor CXCR4 has been implicated in attenuation of bleomycin (BLM)-induced pulmonary fibrosis and carbon tetrachloride (CCl4)-induced hepatic injury. In pulmonary fibrosis, published reports suggest that collagen production in the injured lung is derived from
Tiffany A Katz et al.
Environmental health perspectives, 128(1), 17010-17010 (2020-01-16)
Tributyltin (TBT) is a persistent and bioaccumulative environmental toxicant. Developmental exposure to TBT has been shown to cause fatty liver disease (steatosis), as well as increased adiposity in many species, leading to its characterization as an obesogen. We aimed to
Xiaohuang Lin et al.
Genomics, proteomics & bioinformatics, 18(3), 289-304 (2020-11-02)
Protein lysine methylation is a prevalent post-translational modification (PTM) and plays critical roles in all domains of life. However, its extent and function in photosynthetic organisms are still largely unknown. Cyanobacteria are a large group of prokaryotes that carry out
Afficher tous les articles scientifiques apparentés

Questions

1–5 of 5 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. Is it possible to use the MAK055 for an experiment that involves evaluating samples with and without B6 added? Additionally, will hemoglobin interfere if a blood hemolysate sample is used when assayed with this kit?

    1 answer

    1. The specific experiment in question has not been performed in the lab, and there are no references for the Aspartate Aminotransferase Activity Assay Kit where a similar experiment has been reported. Considering the nature of the assay and the fact that B6 is a co-enzyme of the ASP, necessary for its activity, in principle, the kit will be suitable to evaluate samples treated with B6. It will be necessary to evaluate any possible interferences and optimize user-specific conditions.
      It is possible that hemoglobin will interfere with the assay if hemolyzed blood is tested. Blood is generally not recommended as a sample, unless specifically mentioned in the assay, due to the possible interference as mentioned. Furthermore, the kit has not been tested with blood samples.

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  4. Does the AST Activity Assay Kit (MAK055), which includes a positive control (MAK055F), have a specific species origin? For instance, is it Human AST?

    1 answer

    1. The positive control in MAK055 is derived from porcine heart.

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  5. Do you anticipate phenol red in culture medium having an impact on the colorimetric read out at 450 nm? (I'm thinking there may be some spectral overlap)

    1 answer

    1. Ideally, culture media should be phenol red-free.

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